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PMID: 2152159 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A carboxyl-terminal propeptide is necessary for proper sorting of barley lectin to vacuoles of tobacco.

The Plant cell ·Vol. 2 ·No. 12 ·1990-12-00 ·Pages 1145-55

Bednarek SY, Wilkins TA, Dombrowski JE, Raikhel NV

Abstract

Barley lectin is synthesized as a preproprotein with a glycosylated carboxyl-terminal propeptide (CTPP) that is removed before or concomitant with deposition of the mature protein in vacuoles. Expression of a cDNA clone encoding barley lectin in transformed tobacco plants results in the correct processing, maturation, and accumulation of active barley lectin in vacuoles [Wilkins, T.A., Bednarek, S.Y., and Raikhel, N.V. (1990). Plant Cell 2, 301-313]. The glycan of the propeptide is not essential for vacuolar sorting, but may influence the rate of post-translational processing [Wilkins, T.A., Bednarek, S.Y., and Raikhel, N.V. (1990). Plant Cell 2, 301-313]. To investigate the functional role of the CTPP in processing, assembly, and sorting of barley lectin to vacuoles, a mutant barley lectin cDNA clone lacking the 15-amino acid CTPP was prepared. The CTPP deletion mutant of barley lectin was expressed in tobacco protoplasts, suspension-cultured cells, and transgenic plants. In all three systems, the wild-type barley lectin was sorted to vacuoles, whereas the mutant barley lectin was secreted to the incubation media. Therefore, we conclude that the carboxyl-terminal domain of the barley lectin proprotein is necessary for the efficient sorting of this protein to plant cell vacuoles.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport Golgi Apparatus/metabolism Hordeum/metabolism Lectins/metabolism Models, Biological Molecular Sequence Data Mutagenesis, Site-Directed Plant Lectins Plants, Toxic Protein Precursors/metabolism Protein Sorting Signals/metabolism Sequence Homology Tobacco/metabolism Transformation, Genetic Vacuoles/metabolism
Chemicals
Lectins Plant Lectins Protein Precursors Protein Sorting Signals
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bednarek S Y
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing 48824-1312.
Wilkins T A
Dombrowski J E
Raikhel N V
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.2.12.1145
Published
1990-12-00
Pages
1145-55
Language
English
Region
England
NLM ID
9208688
PMCID
PMC159962
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.2.12.1145 PMC Full Text View journal details
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