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PMID: 2152829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Protection from herpes simplex virus type 1 lethal and latent infections by secreted recombinant glycoprotein B constitutively expressed in human cells with a BK virus episomal vector.

Journal of virology ·Vol. 64 ·No. 1 ·1990-01-00 ·Pages 431-6

Manservigi R, Grossi MP, Gualandri R, Balboni PG, Marchini A, Rotola A, Rimessi P, Di Luca D, Cassai E, Barbanti-Brodano G

Abstract

The herpes simplex virus type 1 (HSV-1) glycoprotein B (gB-1) gene, deleted of 639 nucleotides that encode the transmembrane anchor sequence and reconstructed with the extramembrane and intracytoplasmic domains, was cloned under control of the Rous sarcoma virus long terminal repeat in the episomal replicating vector pRP-RSV, which contains the origin of replication and early region of the human papovavirus BK as well as a cDNA for a mutant mouse dihydrofolate reductase that is resistant to methotrexate. gB-1 (0.15 to 0.25 pg per cell per 24 h) was constitutively secreted into the culture medium of pRP-RSV-gBs-transformed human 293 cells. Treatment of transformed cells with methotrexate at high concentrations (0.6 to 6 microM) increased gB-1 production 10- to 100-fold, because of an amplification of the episomal recombinant. Mice immunized with secreted gB-1 produced HSV-1- and HSV-2-neutralizing antibodies and were protected against HSV-1 lethal, latent, and recurrent infections. Constitutive expression of secreted gB-1 in human cells may establish a system to develop diagnostic material and a subunit vaccine for HSV infections.

MeSH Terms
Animals BK Virus/genetics Blotting, Southern Female Gene Expression Genes, Viral Genetic Vectors Herpes Simplex/immunology,prevention & control Immunization Mice Mice, Inbred BALB C Nucleic Acid Hybridization Plasmids Polyomavirus/genetics Recombinant Proteins/administration & dosage,immunology Simplexvirus/genetics,pathogenicity Viral Envelope Proteins/administration & dosage,genetics,immunology Viral Structural Proteins/genetics
Chemicals
Recombinant Proteins Viral Envelope Proteins Viral Structural Proteins glycoprotein B, Simplexvirus
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Manservigi R
Institute of Microbiology, School of Medicine, University of Ferrara, Italy.
Grossi M P
Gualandri R
Balboni P G
Marchini A
Rotola A
Rimessi P
Di Luca D
Cassai E
Barbanti-Brodano G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.1.431-436.1990
Published
1990-01-00
Pages
431-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249122
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.1.431-436.1990 PMC Full Text View journal details
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