Abstract
The speB gene of Escherichia coli encodes the enzyme agmatine ureohydrolase (AUH). AUH catalyzes the hydrolysis of agmatine to urea and putrescine in one of the two polyamine biosynthetic pathways in E. coli. Sequencing of a 2.97-kilobase-pair fragment of the E. coli chromosome containing speB revealed the presence of three intact open reading frames (ORFs), ORF1 and ORF2 on one strand and ORF3 on the opposite strand, as well as a truncated ORF, ORF4, which terminated 92 kilobase pairs upstream from ORF3. ORF3 contained the coding sequence of the speB gene, as confirmed by complementation analysis. Two ORF3 transcripts were detected: a shorter transcript that included only ORF3 and a longer transcript that included both ORF3 and ORF4. The short transcript was abundantly expressed when the ORF4 sequences were deleted, but when ORF4 and its upstream sequences were present, the polycistronic message predominated and the amount of the monocistronic message was drastically reduced. The promoter from which the shorter transcript was produced contained a TATACT sequence at position -12, but sequences upstream from the -12 position seemed to be irrelevant for promoter activity. The predicted amino acid sequence of AUH contained three regions of high homology to the arginases of yeasts, rats, and humans.
MeSH Terms
Amino Acid Sequence
Animals
Arginase/genetics
Base Sequence
Blotting, Northern
Chromosome Deletion
DNA Transposable Elements
DNA, Bacterial/genetics,isolation & purification
Escherichia coli/enzymology,genetics
Genes
Genes, Bacterial
Humans
Molecular Sequence Data
Nucleic Acid Hybridization
Plasmids
Putrescine/biosynthesis
Recombinant Proteins/metabolism
Restriction Mapping
Sequence Homology, Nucleic Acid
Ureohydrolases/genetics,metabolism
Chemicals
DNA Transposable Elements
DNA, Bacterial
Recombinant Proteins
Ureohydrolases
Arginase
AGMAT protein, human
agmatinase
Putrescine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Szumanski M B
Department of Biochemistry and Nutrition, College of Agriculture and Life Sciences, Blacksburg, Virginia.
Boyle S M
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