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PMID: 2829127 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The presence of five nifH-like sequences in Clostridium pasteurianum: sequence divergence and transcription properties.

Nucleic acids research ·Vol. 16 ·No. 2 ·1988-01-25 ·Pages 439-54

Wang SZ, Chen JS, Johnson JL

Abstract

The nifH gene encodes the iron protein (component II) of the nitrogenase complex. We have previously shown the presence in Clostridium pasteurianum of two nifH-like sequences in addition to the nifH1 gene which codes for a protein identical to the isolated iron protein. In the present study, we report that there are at least five nifH-like sequences in C. pasteurianum. DNA sequencing data indicate that the six nifH (nifH1) and nifH-like (nifH2, nifH3, nifH4, nifH5 and nifH6) sequences are not identical and vary from each other to different extents with sequence identity ranging between 68 to 99.9% within the nifH coding regions. Under normal N2-fixing growth conditions (molybdenum-containing medium), transcripts of nifH1 and most of the nifH-like sequences accumulate. The above results suggest the functioning of more than one "nifH" gene under N2-fixing growth conditions for C. pasteurianum. A common sequence was found around the -100 regions of all nif or nif-like transcription units. Sequences identical to or very similar to the consensus Escherichia coli promoter were found in the -35 and -10 regions.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular Clostridium/genetics DNA Restriction Enzymes Genes, Bacterial Genes, Regulator Molecular Sequence Data Nitrogen Fixation/genetics Regulatory Sequences, Nucleic Acid Transcription, Genetic
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang S Z
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Chen J S
Johnson J L
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.2.439
Published
1988-01-25
Pages
439-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334671
Subset
IM
Databases
GENBANK
X06756, X07472, X07473, X07474, X07475, X07476, X07477
External Links
PubMed source DOI: 10.1093/nar/16.2.439 PMC Full Text View journal details
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