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PMID: 2430949 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and sequence of the gene for ferredoxin I from the cyanobacterium Anabaena sp. strain PCC 7120.

Journal of bacteriology ·Vol. 168 ·No. 3 ·1986-12-00 ·Pages 1265-71

Alam J, Whitaker RA, Krogmann DW, Curtis SE

Abstract

The structural gene for ferredoxin I, petF, from the cyanobacterium Anabaena sp. strain PCC 7120 has been isolated from a recombinant lambda library. Mixtures of tetradecanucleotides and heptadecanucleotides, each containing all possible DNA sequences corresponding to two separate regions of the ferredoxin amino acid sequence, were synthesized and used as hybridization probes to identify a genomic clone containing the coding sequence for the petF gene. The sequence of the entire petF coding region and portions of the 3'- and 5'-flanking regions was determined. The DNA sequence of petF suggests that, in contrast to the nucleus-encoded plant protein, cyanobacterial apoferredoxin is not synthesized as a higher-molecular-weight precursor. The Anabaena petF gene is a single-copy gene. During growth on complete medium it was transcribed into a monocistronic mRNA species of approximately 500 bases that initiated 100 base pairs upstream from the petF coding region.

MeSH Terms
Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence Cyanobacteria/genetics Ferredoxins/genetics Genes Genes, Bacterial RNA, Bacterial/biosynthesis RNA, Messenger/biosynthesis Recombinant Proteins/genetics Species Specificity
Chemicals
Ferredoxins RNA, Bacterial RNA, Messenger Recombinant Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Alam J
Whitaker R A
Krogmann D W
Curtis S E
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.168.3.1265-1271.1986
Published
1986-12-00
Pages
1265-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213632
Subset
IM
Grants
NIGMS NIH HHS · GM2766 · United States
Databases
GENBANK
M14737
External Links
PubMed source DOI: 10.1128/jb.168.3.1265-1271.1986 PMC Full Text View journal details
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