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PMID: 2153660 Published · ppublish English Journal Article

Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase.

Journal of bacteriology ·Vol. 172 ·No. 2 ·1990-02-00 ·Pages 802-7

Pradel E, Marck C, Boquet PL

Abstract

The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a calculated Mr of 43,514. Its promoter was defined by primer extension of the mRNA made in vivo. Like many genes under positive control, its -35 promoter region does not match the consensus. The agp gene is both preceded and followed by transcription termination signals, so it appears to be transcribed as a single unit.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Escherichia coli Proteins Genes, Bacterial Molecular Sequence Data Phosphoric Monoester Hydrolases/genetics Plasmids Promoter Regions, Genetic RNA, Messenger/genetics Restriction Mapping Transcription, Genetic
Chemicals
DNA, Bacterial Escherichia coli Proteins RNA, Messenger agp protein, E coli Phosphoric Monoester Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pradel E
Departement de Biologie, Centre d'Etudes Nucléaires de Saclay, Gif-sur-Yvette, France.
Marck C
Boquet P L
References (35)
35 references, click to expand
  1. Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli.
    Gene. 1986;44(1):121-5 PMID: 3533724
  2. Mapping of the Escherichia coli acid glucose-1-phosphatase gene agp and analysis of its expression in vivo by use of an agp-phoA protein fusion.
    J Bacteriol. 1989 Jun;171(6):3511-7 PMID: 2542226
  3. Studies on the uptake of hexose phosphates. 3. Mechanism of uptake of glucose 1-phosphate in Escherichia coli.
    J Biol Chem. 1971 May 10;246(9):2891-7 PMID: 4324342
  4. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  5. Genetic location of the gene (ush) specifying periplasmic uridine 5'-diphosphate glucose hydrolase (5'-nucleotidase) in Escherichia coli K-12.
    J Bacteriol. 1976 Oct;128(1):487-9 PMID: 789351
  6. Resolution and purification of three periplasmic phosphatases of Salmonella typhimurium.
    J Bacteriol. 1977 Apr;130(1):399-410 PMID: 192712
  7. Properties of two phosphatases and a cyclic phosphodiesterase of Salmonella typhimurium.
    J Bacteriol. 1977 Apr;130(1):411-9 PMID: 15982
  8. Regulation of two phosphatases and a cyclic phosphodiesterase of Salmonella typhimurium.
    J Bacteriol. 1977 Apr;130(1):420-8 PMID: 192713
  9. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  10. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  11. Physiological function of periplasmic hexose phosphatase in Salmonella typhimurium.
    J Bacteriol. 1980 Mar;141(3):1474-7 PMID: 6245072
  12. Isolation of Escherichia coli mutants (cpdB) deficient in periplasmic 2':3'-cyclic phosphodiesterase and genetic mapping of the cpdB locus.
    J Gen Microbiol. 1980 Jul;119(1):31-4 PMID: 6251162
  13. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  14. Cloning and characterization of the Escherichia coli gene coding for alkaline phosphatase.
    J Bacteriol. 1981 May;146(2):660-7 PMID: 6260755
  15. Constitutive function of a positively regulated promoter reveals new sequences essential for activity.
    J Biol Chem. 1987 May 5;262(13):6389-95 PMID: 3032964
  16. Molecular cloning of the gene (ush) from Escherichia coli specifying periplasmic UDP-sugar hydrolase (5'-nucleotidase).
    Gene. 1980 Dec;12(3-4):281-6 PMID: 6265322
  17. Amino acid sequence of Escherichia coli alkaline phosphatase.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3473-7 PMID: 7022451
  18. Evidence for two functional gal promoters in intact Escherichia coli cells.
    J Biol Chem. 1981 Nov 25;256(22):11905-10 PMID: 6271763
  19. Patterns of amino acids near signal-sequence cleavage sites.
    Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
  20. Characterization of the ush gene of Escherichia coli and its protein products.
    Gene. 1983 Nov;25(2-3):343-53 PMID: 6363215
  21. In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1480-3 PMID: 6324195
  22. A computer algorithm for testing potential prokaryotic terminators.
    Nucleic Acids Res. 1984 May 25;12(10):4411-27 PMID: 6374619
  23. Positive control of transcription initiation in bacteria.
    Annu Rev Genet. 1984;18:173-206 PMID: 6084980
  24. DNA sequences at the ends of the genome of bacteriophage Mu essential for transposition.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):2087-91 PMID: 2984681
  25. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  26. Identification of the gene appA for the acid phosphatase (pH optimum 2.5) of Escherichia coli.
    Mol Gen Genet. 1985;200(1):68-73 PMID: 2993816
  27. Nucleotide sequence and transcriptional analysis of the E. coli ushA gene, encoding periplasmic UDP-sugar hydrolase (5'-nucleotidase): regulation of the ushA gene, and the signal sequence of its encoded protein product.
    Nucleic Acids Res. 1986 May 27;14(10):4325-42 PMID: 3012467
  28. Analysis of E. coli promoter sequences.
    Nucleic Acids Res. 1987 Mar 11;15(5):2343-61 PMID: 3550697
  29. Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro.
    J Bacteriol. 1981 May;146(2):668-75 PMID: 6260756
  30. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
  31. The structure of the promoter and amino terminal region of the pH 2.5 acid phosphatase structural gene (appA) of E. coli: a negative control of transcription mediated by cyclic AMP.
    Biochimie. 1987 Mar;69(3):215-21 PMID: 3038201
  32. 'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers.
    Nucleic Acids Res. 1988 Mar 11;16(5):1829-36 PMID: 2832831
  33. Structures of the promoter and operator of the glpD gene encoding aerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12.
    J Bacteriol. 1988 Sep;170(9):4209-15 PMID: 3045087
  34. Acid phosphatases of Escherichia coli: molecular cloning and analysis of agp, the structural gene for a periplasmic acid glucose phosphatase.
    J Bacteriol. 1988 Oct;170(10):4916-23 PMID: 2844729
  35. Use of TnphoA to detect genes for exported proteins in Escherichia coli: identification of the plasmid-encoded gene for a periplasmic acid phosphatase.
    J Bacteriol. 1987 Apr;169(4):1663-9 PMID: 3031017
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.2.802-807.1990
Published
1990-02-00
Pages
802-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208509
Subset
IM
Databases
GENBANK
M33807
External Links
PubMed source DOI: 10.1128/jb.172.2.802-807.1990 PMC Full Text View journal details
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