Comparative studies of five heat-labile toxic products of Escherichia coli.

Infection and immunity ·Vol. 22 ·No. 3 ·1978-12-00 ·Pages 644-8

Konowalchuk J, Dickie N, Stavric S, Speirs JI

Abstract

Five heat-labile, partially purified toxic products of Escherichia coli were distinguished by isoelectric focusing, molecular weight, and neutralization with homologous and heterologous antisera. Only two affected the morphology of Y-1 cells, induced fluid accumulation in rabbit ileal loops, and stimulated production of cyclic AMP in Vero cells; these two did not cross-neutralize and only one showed cross-neutralization with cholera antitoxin. The remaining three products were cytotoxic for Vero cells.

MeSH Terms

Bacterial Toxins/analysis,biosynthesis Body Water/metabolism Cell Line Cyclic AMP/metabolism Enterotoxins/analysis,biosynthesis Escherichia coli/analysis,metabolism Hot Temperature Isoelectric Point Neutralization Tests

Chemicals

Bacterial Toxins Enterotoxins Cyclic AMP

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Konowalchuk J

Dickie N

Stavric S

Speirs J I

References (10)

10 references, click to expand

Heat-labile enterotoxins of Escherichia coli.
Toxicon. 1977;15(6):511-9 PMID: 333655
Factors influencing heat-labile Escherichia coli enterotoxin activity.
Infect Immun. 1976 Aug;14(2):383-8 PMID: 9363
Properties of an Escherichia coli cytotoxin.
Infect Immun. 1978 May;20(2):575-7 PMID: 208977
Vero response to a cytotoxin of Escherichia coli.
Infect Immun. 1977 Dec;18(3):775-9 PMID: 338490
The titration of cholera toxin and antitoxin in the rabbit ileal loop.
J Infect Dis. 1966 Dec;116(5):606-14 PMID: 5957267
Purification and some properties of a heat-labile enterotoxin from escherichia coli.
Zentralbl Bakteriol Orig A. 1974;229(2):190-204 PMID: 4155864
A protein binding assay for adenosine 3':5'-cyclic monophosphate.
Proc Natl Acad Sci U S A. 1970 Sep;67(1):305-12 PMID: 4318781
Classification of enterotoxins on the basis of activity in cell culture.
J Infect Dis. 1975 Jan;131(1):58-63 PMID: 162926
Differences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins of Escherichia coli.
Infect Immun. 1973 Jun;7(6):873-80 PMID: 4577415
Partial purification and characterization of a heat-labile enterotoxin of Escherichia coli.
Infect Immun. 1976 Jun;13(6):1710-20 PMID: 786881

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.22.3.644-648.1978

Published

1978-12-00

Pages

644-8

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC422208

Subset

External Links

PubMed source DOI: 10.1128/iai.22.3.644-648.1978 PMC Full Text View journal details

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Comparative studies of five heat-labile toxic products of Escherichia coli.

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