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PMID: 2158108 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transposition rates of movable genetic elements in Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 8 ·1990-04-00 ·Pages 3248-52

Harada K, Yukuhiro K, Mukai T

Abstract

A considerable proportion of visible mutations is reported to be caused by the insertion of mobile genetic elements in Drosophila and other organisms. We estimated transposition rates of some Drosophila mobile elements by using the lines AW and JH in which spontaneous mutations have been accumulated independently for about 400 generations. Occupied sites of the mobile elements were detected by in situ hybridization on the salivary gland chromosomes sampled from 40 AW and 30 JH lines. The rates of insertion and excision of the copia and two copia-like elements, 412 and 17.6, are very low: Insertions occurred at up to 10(-3) per second chromosome per generation (17.6) and excision occurred at about 10(-5) per site per generation (copia and 412). Insertions of the I and hobo elements occurred much more frequently. These estimates are not only important for assessing the actual rate of various types of mutations but also for developing an evolutionary theory of mobile elements themselves.

MeSH Terms
Animals Biological Evolution Chromosome Mapping Crosses, Genetic DNA Transposable Elements Drosophila melanogaster/genetics Female Male Mutation Plasmids Salivary Glands/physiology
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Harada K
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Yukuhiro K
Mukai T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.8.3248
Published
1990-04-00
Pages
3248-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53873
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.8.3248 PMC Full Text View journal details
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