Home LiteratureArticle Details
PMID: 2987642 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Excision of copia element in a revertant of the white-apricot mutation of Drosophila melanogaster leaves behind one long-terminal repeat.

Molecular & general genetics : MGG ·Vol. 199 ·No. 1 ·1985-00-00 ·Pages 1-6

Carbonare BD, Gehring WJ

Abstract

The spontaneous mutation white-apricot (wa) in Drosophila melanogaster has a considerably lighter eye colour than the wild-type, and is caused by the insertion of a copia transposable element into a small intron of the white gene. We have analyzed an X-ray induced wa revertant (waR59K1), whose eye pigmentation is incompletely restored, by in situ hybridization, Southern blotting and sequencing analysis. At the site where copia had originally inserted, we found one long terminal repeat of copia, flanked by a 5 bp duplication with the same polarity as the direction of transcription of the white locus. These results suggest that the wa reversion is due to homologous recombination between the two long terminal repeats of copia.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Transposable Elements Drosophila melanogaster/genetics Eye Color Mutation/radiation effects Nucleic Acid Hybridization Recombination, Genetic Repetitive Sequences, Nucleic Acid
Chemicals
DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Carbonare B D
Gehring W J
References (40)
40 references, click to expand
  1. Physical map of the white locus of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):564-8 PMID: 6281775
  2. Insertion of the Drosophila transposable element copia generates a 5 base pair duplication.
    Cell. 1980 Sep;21(2):575-9 PMID: 6250725
  3. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method.
    Cell. 1981 Sep;25(3):693-704 PMID: 6269753
  4. The U3 portion of feline leukemia virus DNA identifies horizontally acquired proviruses in leukemic cats.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7778-82 PMID: 6278488
  5. Transformation-defective mutants of feline sarcoma virus which express a product of the viral src gene.
    J Virol. 1980 Jul;35(1):129-40 PMID: 6251261
  6. Evidence for transposition of dispersed repetitive DNA families in yeast.
    Cell. 1979 Apr;16(4):739-51 PMID: 378399
  7. In vitro packaging of lambda Dam vectors and their use in cloning DNA fragments.
    Methods Enzymol. 1979;68:281-98 PMID: 232217
  8. Characterization of the yeast mobile element Ty1.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:609-17 PMID: 6266754
  9. Dilute (d) coat colour mutation of DBA/2J mice is associated with the site of integration of an ecotropic MuLV genome.
    Nature. 1981 Oct 1;293(5831):370-4 PMID: 6268990
  10. Close relationship between the long terminal repeats of avian leukosis-sarcoma virus and copia-like movable genetic elements of Drosophila.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3193-7 PMID: 6304696
  11. Novel bacteriophage lambda cloning vector.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5172-6 PMID: 6254062
  12. DNA rearrangements associated with a transposable element in yeast.
    Cell. 1980 Aug;21(1):239-49 PMID: 6250713
  13. Immunological method for mapping genes on Drosophila polytene chromosomes.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4381-5 PMID: 6812046
  14. Molecular Genetics of the Bithorax Complex in Drosophila melanogaster.
    Science. 1983 Jul 1;221(4605):23-9 PMID: 17737996
  15. Transposable elements.
    Cell. 1980 Jul;20(3):579-95 PMID: 6251969
  16. Terminal repeats of the Drosophila transposable element copia: nucleotide sequence and genomic organization.
    Cell. 1980 Sep;21(2):581-8 PMID: 6250726
  17. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  18. Molecular cloning of the white locus region of Drosophila melanogaster using a large transposable element.
    EMBO J. 1982;1(1):93-8 PMID: 16453411
  19. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  20. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  21. Extrachromosomal circular copies of the eukaryotic transposable element copia in cultured Drosophila cells.
    Nature. 1981 Aug 13;292(5824):591-5 PMID: 6265802
  22. Nucleotide sequence of terminal repeats of 412 transposable elements of Drosophila melanogaster. A similarity to proviral long terminal repeats and its implications for the mechanism of transposition.
    J Mol Biol. 1981 Dec 25;153(4):897-915 PMID: 6283088
  23. Unusual structure of the FB family of transposable elements in Drosophila.
    Cell. 1981 Jun;24(3):753-63 PMID: 6265101
  24. Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication.
    Nature. 1980 Jul 24;286(5771):352-6 PMID: 6250062
  25. Transcription of the Drosophila white locus and some of its mutants.
    EMBO J. 1984 Mar;3(3):563-8 PMID: 6425054
  26. Mobile dispersed genetic element MDG1 of Drosophila melanogaster: nucleotide sequence of long terminal repeats.
    Nucleic Acids Res. 1981 Jul 24;9(14):3451-64 PMID: 6269083
  27. A transposable element inserted just 5' to a Drosophila glue protein gene alters gene expression and chromatin structure.
    Cell. 1983 Aug;34(1):75-84 PMID: 6309414
  28. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
    Anal Biochem. 1980 Nov 15;109(1):123-9 PMID: 6162401
  29. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
    Cell. 1974 Dec;3(4):315-25 PMID: 4216403
  30. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations.
    Cell. 1982 Jul;29(3):987-94 PMID: 6295640
  31. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome.
    Cell. 1983 Aug;34(1):25-35 PMID: 6309410
  32. A copy of the copia transposable element is very tightly linked to the Wa allele at the white locus of D. melanogaster.
    Cell. 1981 Sep;25(3):705-11 PMID: 6269754
  33. Repeated gene families in Drosophila melanogaster.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1053-63 PMID: 98263
  34. Effects of transposable element insertions on RNA encoded by the white gene of Drosophila.
    Cell. 1984 Sep;38(2):471-81 PMID: 6088083
  35. Structure of the Drosophila mutable allele, white-crimson, and its white-ivory and wild-type derivatives.
    Cell. 1982 Aug;30(1):71-9 PMID: 6290083
  36. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  37. Isolation of a hybrid plasmid with homologous sequences to a transposing element of Drosophila melanogaster.
    Cell. 1980 Apr;19(4):897-904 PMID: 6247074
  38. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  39. A physical map of the DNA regions flanking the rabbit beta-globin gene.
    Cell. 1977 Oct;12(2):429-39 PMID: 912752
  40. Nucleic acid hybridization to the DNA of cytological preparations.
    Methods Cell Biol. 1975;10:1-16 PMID: 1102840
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327501
Published
1985-00-00
Pages
1-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327501
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2