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PMID: 2170328 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome.

Journal of bacteriology ·Vol. 172 ·No. 10 ·1990-10-00 ·Pages 5828-36

Rong L, Karcher SJ, O'Neal K, Hawes MC, Yerkes CD, Jayaswal RK, Hallberg CA, Gelvin SB

Abstract

We used the transposon Mu dI1681 to identify genes on the Agrobacterium tumefaciens chromosome that are inducible by extracts from carrot roots. One such locus (picA, for plant inducible chromosomal), harbored by A. tumefaciens At156, was inducible 10- to 50-fold by these extracts. Mutation of picA had no detectable effect upon bacterial growth or virulence under laboratory assay conditions. However, A. tumefaciens cells harboring a mutated picA locus aggregated into long "ropes" when incubated with pea root tip cells. Such aggregation was not displayed by the parental strain A. tumefaciens A136. A preliminary characterization of the inducing compound in the carrot root extract suggests that the active substance is an acidic polysaccharide that is most likely derived from the pectic portion of the plant cell wall.

MeSH Terms
Chromosomes, Bacterial Cloning, Molecular Cosmids DNA Transposable Elements Fabaceae/physiology Gene Expression Regulation, Bacterial Genes, Bacterial Mutation Plant Physiological Phenomena Plants, Medicinal Restriction Mapping Rhizobium/genetics
Chemicals
DNA Transposable Elements
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Rong L
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Karcher S J
O'Neal K
Hawes M C
Yerkes C D
Jayaswal R K
Hallberg C A
Gelvin S B
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.10.5828-5836.1990
Published
1990-10-00
Pages
5828-36
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC526900
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.10.5828-5836.1990 PMC Full Text View journal details
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