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PMID: 6315684 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Suicide plasmid vehicles for insertion mutagenesis in Rhizobium meliloti and related bacteria.

Journal of bacteriology ·Vol. 156 ·No. 3 ·1983-12-00 ·Pages 1292-300

Selvaraj G, Iyer VN

Abstract

We describe the construction and use of a set of plasmid vectors of the transposons Tn1, Tn5, and Tn9 that are suicidal in Rhizobium species and therefore suitable for mutagenesis with these three transposons. The vectors are composed of the p15A replicon which functions in Escherichia coli but not in Rhizobium species and a region encoding the N type of bacterial conjugation system which is very efficient in matings between E. coli and Rhizobium species. The usefulness of the vectors has been most extensively assessed in Rhizobium meliloti. It is likely that they will be useful for mutagenesis and genome manipulation in other bacteria as well.

MeSH Terms
Conjugation, Genetic DNA Transposable Elements Escherichia coli/genetics Genetic Vectors Mutation Plasmids Replicon Rhizobium/genetics
Chemicals
DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Selvaraj G
Iyer V N
References (31)
31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.3.1292-1300.1983
Published
1983-12-00
Pages
1292-300
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217980
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.156.3.1292-1300.1983 PMC Full Text View journal details
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