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PMID: 2172978 Published · ppublish English Comparative Study Journal Article

Related fibroblast growth factor receptor genes exist in the human genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 20 ·1990-10-00 ·Pages 8180-4

Houssaint E, Blanquet PR, Champion-Arnaud P, Gesnel MC, Torriglia A, Courtois Y, Breathnach R

Abstract

We have isolated, from a human tumor cDNA library, a gene encoding a putative receptor-like protein-tyrosine kinase that we call TK14. The amino acid sequence of the TK14 protein is closely related to the available partial sequence of the mouse protein bek, and more distantly related to the sequences of a chicken basic fibroblast growth factor receptor (73% sequence homology) and the apparent human equivalent of this receptor, the FLG protein (encoded by the fms-like tyrosine kinase gene). Overexpression of the TK14 protein by transfection of COS-1 cells with the corresponding cDNA in a simian virus 40-based expression vector leads to the appearance of new cell-surface binding sites for both acidic and basic fibroblast growth factors. This has been demonstrated by specific binding assays and chemical cross-linking experiments using 125I-labeled growth factors. It appears, therefore, that the human genome contains at least two distinct genes, for TK14 and FLG, that code for related fibroblast growth factor receptors.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA/genetics,isolation & purification Fibroblast Growth Factors/metabolism Filaggrin Proteins Gene Library Genome, Human Humans Kinetics Molecular Sequence Data Oligonucleotide Probes Receptors, Cell Surface/genetics,metabolism Receptors, Fibroblast Growth Factor Sequence Homology, Nucleic Acid Transfection
Chemicals
FLG protein, human Filaggrin Proteins Oligonucleotide Probes Receptors, Cell Surface Receptors, Fibroblast Growth Factor Fibroblast Growth Factors DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Houssaint E
Faculté des Sciences et des Techniques, Université de Nantes, France.
Blanquet P R
Champion-Arnaud P
Gesnel M C
Torriglia A
Courtois Y
Breathnach R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.20.8180
Published
1990-10-00
Pages
8180-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54916
Subset
IM
Databases
GENBANK
M37715, M55614
External Links
PubMed source DOI: 10.1073/pnas.87.20.8180 PMC Full Text View journal details
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