Home LiteratureArticle Details
PMID: 2172984 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Host genes that influence transposition in yeast: the abundance of a rare tRNA regulates Ty1 transposition frequency.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 21 ·1990-11-00 ·Pages 8360-4

Xu H, Boeke JD

Abstract

Genetic screening of a yeast genomic library in a high-copy-number vector identified the normally single copy tRNA(CCU Arg) gene as one of the genes and reduces Ty1 transposition frequency when overexpressed. Immunoblot analyses of Ty1-encoded proteins indicate an inverse correlation between the copy number of the tRNA gene and the production of the TYB protein. Thus, Ty1 transposition frequency is apparently regulated by the level of tRNA(CCU Arg) in yeast cells.

MeSH Terms
Codon/genetics DNA Transposable Elements Fungal Proteins/genetics,isolation & purification Gene Expression Regulation, Fungal Genes, Fungal Plasmids RNA, Transfer, Arg/genetics Saccharomyces cerevisiae/genetics
Chemicals
Codon DNA Transposable Elements Fungal Proteins RNA, Transfer, Arg
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Xu H
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Boeke J D
References (23)
23 references, click to expand
  1. Localization of sequences required in cis for yeast Ty1 element transposition near the long terminal repeats: analysis of mini-Ty1 elements.
    Mol Cell Biol. 1990 Jun;10(6):2695-702 PMID: 2160583
  2. Sequence and structural features associated with translational initiator regions in yeast--a review.
    Gene. 1987;59(1):1-18 PMID: 3325335
  3. Yeast/E. coli shuttle vectors with multiple unique restriction sites.
    Yeast. 1986 Sep;2(3):163-7 PMID: 3333305
  4. Processing of TY1 proteins and formation of Ty1 virus-like particles in Saccharomyces cerevisiae.
    Mol Gen Genet. 1987 May;207(2-3):421-9 PMID: 3039300
  5. Nucleotide sequence of a yeast Ty element: evidence for an unusual mechanism of gene expression.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2829-33 PMID: 2581255
  6. Expression strategies of the yeast retrotransposon Ty: a short sequence directs ribosomal frameshifting.
    Nucleic Acids Res. 1986 Sep 11;14(17):7001-16 PMID: 3020502
  7. Ribosomal frameshifting in the yeast retrotransposon Ty: tRNAs induce slippage on a 7 nucleotide minimal site.
    Cell. 1990 Jul 27;62(2):339-52 PMID: 2164889
  8. A retrovirus-like strategy for expression of a fusion protein encoded by yeast transposon Ty1.
    Nature. 1985 Jan 17-23;313(5999):243-6 PMID: 2982101
  9. The functions and relationships of Ty-VLP proteins in yeast reflect those of mammalian retroviral proteins.
    Cell. 1987 Apr 10;49(1):111-9 PMID: 3030564
  10. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  11. Nucleotide sequence and characteristics of a Ty element from yeast.
    Nucleic Acids Res. 1985 Apr 25;13(8):2745-58 PMID: 2987866
  12. The Saccharomyces cerevisiae genome contains functional and nonfunctional copies of transposon Ty1.
    Mol Cell Biol. 1988 Apr;8(4):1432-42 PMID: 2837641
  13. Functional organization of the retrotransposon Ty from Saccharomyces cerevisiae: Ty protease is required for transposition.
    Mol Cell Biol. 1988 Apr;8(4):1421-31 PMID: 2454391
  14. RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2432-6 PMID: 6189122
  15. The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles: cell-free Ty1 transposition.
    Cell. 1988 Sep 23;54(7):955-66 PMID: 2843295
  16. Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Mar;7(3):1198-207 PMID: 3550432
  17. Ty elements transpose through an RNA intermediate.
    Cell. 1985 Mar;40(3):491-500 PMID: 2982495
  18. High-frequency deletion between homologous sequences during retrotransposition of Ty elements in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8553-7 PMID: 2825195
  19. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
    Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
  20. A general method for the chromosomal amplification of genes in yeast.
    Science. 1988 Jan 15;239(4837):280-2 PMID: 2827308
  21. Efficient translational frameshifting occurs within a conserved sequence of the overlap between the two genes of a yeast Ty1 transposon.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6816-20 PMID: 2842793
  22. Delta sequences in the 5' non-coding region of yeast tRNA genes.
    EMBO J. 1983;2(4):583-91 PMID: 16453444
  23. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.21.8360
Published
1990-11-00
Pages
8360-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54955
Subset
IM
Grants
NIGMS NIH HHS · GM 36481 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.87.21.8360 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2