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PMID: 2176150 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes.

The EMBO journal ·Vol. 9 ·No. 13 ·1990-12-00 ·Pages 4339-46

Sneddon AA, Cohen PT, Stark MJ

Abstract

Two genes (PPH21 and PPH22) encoding the yeast homologues of protein serine-threonine phosphatase 2A have been cloned from a Saccharomyces cerevisiae genomic library using a rabbit protein phosphatase 2A cDNA as a hybridization probe. The PPH genes are genetically linked on chromosome IV and are predicted to encode polypeptides each with 74% amino acid sequence identity to rabbit type 2A protein phosphatase, indicating once again the extraordinarily high degree of sequence conservation shown by protein-phosphatases from different species. The two PPH genes show less than 10% amino acid sequence divergence from each other and while disruption of either PPH gene alone is without any major effect, the double disruption is lethal. This indicates that protein phosphatase 2A activity is an essential cellular function in yeast. Measurement of type 2A protein phosphatase activity in yeast strains lacking one or other of the genes indicates that they account for most, if not all, protein phosphatase 2A activity in the cell.

MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence DNA, Fungal/chemistry Genes, Fungal Molecular Sequence Data Phosphoprotein Phosphatases/biosynthesis,genetics Protein Phosphatase 2 Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Xenopus
Chemicals
DNA, Fungal Phosphoprotein Phosphatases Protein Phosphatase 2
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sneddon A A
Department of Biochemistry, The University, Dundee, UK.
Cohen P T
Stark M J
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30 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-12-00
Pages
4339-46
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552220
Subset
IM
Databases
GENBANK
X56261, X56262
External Links
PubMed source PMC Full Text View journal details
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