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PMID: 2176155 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Perturbation of the ubiquitin system causes leaf curling, vascular tissue alterations and necrotic lesions in a higher plant.

The EMBO journal ·Vol. 9 ·No. 13 ·1990-12-00 ·Pages 4543-9

Bachmair A, Becker F, Masterson RV, Schell J

Abstract

A ubiquitin variant with Lys48 changed to Arg acts in vitro as an inhibitor of ubiquitin dependent protein degradation. To assess the role of this proteolytic pathway in the life cycle of plants, we expressed the ubiquitin variant in Nicotiana tabacum. Expression of variant mono- or polyubiquitin leads to marked abnormalities in vascular tissue. In addition, overexpression of variant polyubiquitin induces discrete lesions on leaves. This indicates that perturbations of the ubiquitin system can induce a programmed necrotic response in plants.

MeSH Terms
Arginine/genetics Base Sequence Gene Expression Genetic Variation Lysine/genetics Molecular Sequence Data Plant Diseases Plants, Toxic Protein Denaturation RNA, Messenger/metabolism Sensitivity and Specificity Tobacco/genetics,growth & development Ubiquitins/analogs & derivatives,biosynthesis,genetics
Chemicals
RNA, Messenger Ubiquitins ubiquitin R-48 Arginine Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bachmair A
Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
Becker F
Masterson R V
Schell J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-12-00
Pages
4543-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552251
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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