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PMID: 2987683 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Ubiquitin is a heat shock protein in chicken embryo fibroblasts.

Molecular and cellular biology ·Vol. 5 ·No. 5 ·1985-05-00 ·Pages 949-56

Bond U, Schlesinger MJ

Abstract

Clones containing heat-inducible mRNA sequences were selected from a cDNA library prepared from polyadenylated RNA isolated from heat-shocked chicken embryo fibroblasts. One recombinant DNA clone, designated clone 7, hybridized to a 1.2-kilobase RNA that was present in normal cells and increased fivefold during heat shock. Clone 7 also hybridized to an RNA species of 1.7 kilobases that was present exclusively in heat-shocked cells. In vitro translation of mRNA hybrid selected from clone 7 produced a protein product with a molecular weight of approximately 8,000. Increased synthesis of a protein of similar size was detected in chicken embryo fibroblasts after heat shock. DNA sequence analysis of clone 7 indicated its protein product has amino acid sequences identical to bovine ubiquitin. In addition, clone 7 contains tandem copies of the ubiquitin sequences contiguous to each other with no untranslated sequences between them. We discuss some possible roles for ubiquitin in the heat shock response.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cells, Cultured Chick Embryo Chromosome Mapping DNA/genetics DNA Restriction Enzymes Heat-Shock Proteins/genetics High Mobility Group Proteins/genetics Hot Temperature Protein Biosynthesis RNA, Messenger/genetics Ubiquitins/genetics
Chemicals
Heat-Shock Proteins High Mobility Group Proteins RNA, Messenger Ubiquitins DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bond U
Schlesinger M J
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.5.949-956.1985
Published
1985-05-00
Pages
949-56
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366809
Subset
IM
Databases
GENBANK
M11100
External Links
PubMed source DOI: 10.1128/mcb.5.5.949-956.1985 PMC Full Text View journal details
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