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PMID: 265581 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 3 ·1977-03-00 ·Pages 864-8

Goldknopf IL, Busch H

Abstract

Chromosomal protein A24 has a unique structure inasmuch as it contains histone 2A and a nonhistone polypeptide the sequence of which has been partially determined. Comparative analysis of the ninhydrin-insensitive amino-terminal tryptic peptides of protein A24 and histone 2A and a quantitative analysis of their carboxyl-terminal amino acid indicated that protein A24 has two amino termini and one carboxyl terminus. The amino acid sequence analysis of tryptic peptide 17' of protein A24: (see text) showed it contains tryptic peptide 17 of histone 2A, Lys-Thr-Glu-Ser-His-His-Lys. Lysine 119, the amino terminus of this peptide, which is derived from the histone 2A portion of protein A24, is linked by an isopeptide bond to the carboxyl group of a glycine residue. Accordingly, the branched structure of protein A24 proposed is: (see text).

MeSH Terms
Amino Acid Sequence Animals Cattle Chromosomal Proteins, Non-Histone Histones Peptides/analysis Thymus Gland Trypsin
Chemicals
Chromosomal Proteins, Non-Histone Histones Peptides Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goldknopf I L
Busch H
References (31)
31 references, click to expand
  1. Isolation of acetylpeptide from enzymic digests of TMV-protein.
    Biochim Biophys Acta. 1958 Apr;28(1):184-91 PMID: 13535695
  2. The estimation of maleic hydrazide by paper chromatography.
    Can J Biochem Physiol. 1958 Jan;36(1):71-4 PMID: 13500244
  3. The NH2- and COOH-terminal amino acid sequence of nuclear protein A24.
    J Biol Chem. 1976 Oct 10;251(19):5901-3 PMID: 972147
  4. Chromosomal proteins and chromatin structure.
    Annu Rev Biochem. 1975;44:725-74 PMID: 1094921
  5. Spheroid chromatin units (v bodies).
    Science. 1974 Jan 25;183(4122):330-2 PMID: 4128918
  6. The identification of isopeptide crosslinks in insoluble fibrin.
    Biochem Biophys Res Commun. 1968 Feb 26;30(4):356-62 PMID: 5637041
  7. Analysis of DNA of isolated chromatin subunits.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3978-82 PMID: 1060078
  8. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  9. Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1316-20 PMID: 4351171
  10. The complete amino acid sequence of ubiquitin, an adenylate cyclase stimulating polypeptide probably universal in living cells.
    Biochemistry. 1975 May 20;14(10):2214-8 PMID: 1170880
  11. Amino acid sequence of the center of the arginine-lysine-rich histone from calf thymus. The total sequence.
    J Biol Chem. 1972 Oct 10;247(19):6018-23 PMID: 4568599
  12. Amino acid sequence of the COOH-terminal portion of the arginine-lysine-rich histone of calf thymus.
    J Biol Chem. 1972 Jun 10;247(11):3589-91 PMID: 4555425
  13. The dynamic state of liver nucleolar proteins as reflected by their changes during administration of thioacetamide.
    Life Sci. 1974 May 16;14(10):1835-45 PMID: 4847790
  14. Chromatin structure: a repeating unit of histones and DNA.
    Science. 1974 May 24;184(4139):868-71 PMID: 4825889
  15. Chromatin structure; oligomers of the histones.
    Science. 1974 May 24;184(4139):865-8 PMID: 4825888
  16. Homology of the amino terminal sequences of the AL and GAR calf thymus histones.
    Physiol Chem Phys. 1972;4(1):10-6 PMID: 4667695
  17. The amino terminal sequence of calf thymus histone 3.
    Biochem Biophys Res Commun. 1972 Jan 14;46(1):50-5 PMID: 5061679
  18. [Cross-link in fibrin polymerized by factor 13: epsilon-(gamma-glutamyl)lysine].
    Science. 1968 May 24;160(3830):892-3 PMID: 4967475
  19. The carboxyl-terminal sequence of porcine pepsin.
    J Biol Chem. 1967 Apr 25;242(8):1833-7 PMID: 5337592
  20. Studies of the formation of peptide cross-links in the cell wall peptidoglycan of Streptococcus faecalis.
    J Biol Chem. 1975 Sep 10;250(17):6806-16 PMID: 808547
  21. Presence of messenger specifying sequences in the DNA of chromatin subunits.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1572-5 PMID: 1064026
  22. A class of chromatin particles associated with nonhistone proteins.
    Biochemistry. 1976 Aug 10;15(16):3510-5 PMID: 952874
  23. Isolation of peptides containing citrulline and the cross-link, epsilon-(gamma-glutamyl)lysine, from hair medulla protein.
    Biochim Biophys Acta. 1976 Mar 18;427(1):315-24 PMID: 1260005
  24. Changes in nucleolar proteins and their phosphorylation patterns during liver regeneration.
    J Biol Chem. 1975 Aug 10;250(15):5921-5 PMID: 1150666
  25. Remarkable similarities of peptide fingerprints of histone 2A and nonhistone chromosomal protein A24.
    Biochem Biophys Res Commun. 1975 Aug 4;65(3):951-60 PMID: 1156427
  26. Isolation and characterization of protein A24, a "histone-like" non-histone chromosomal protein.
    J Biol Chem. 1975 Sep 25;250(18):7182-7 PMID: 1165239
  27. Reaction of peptides with fluorescamine on paper after chromatography or electrophoresis.
    Anal Biochem. 1975 May 12;65(1-2):281-92 PMID: 1130680
  28. Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac.
    Biochemistry. 1971 Dec 21;10(26):4912-21 PMID: 5134536
  29. A large scale procedure for isolation of the glycine-rich, arginine-rich histone and the arginine-rich, lysine-rich histone in a highly purified form.
    J Biol Chem. 1968 Apr 25;243(8):2038-47 PMID: 5646494
  30. N-Terminal acetyl-peptides from two calf thymus histones.
    Biochem J. 1968 Mar;107(2):135-8 PMID: 5641869
  31. Studies on adenosine triphosphate transphophorylases. V. Studies on the polypeptide chains of the crystalline adenosine triphosphate-creatine transphosphorylase from rabbit skeletal muscle.
    Biochemistry. 1967 Oct;6(10):3204-27 PMID: 6056983
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.3.864
Published
1977-03-00
Pages
864-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430507
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.3.864 PMC Full Text View journal details
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