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PMID: 6275256 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ubiquitin - protein conjugates.

Molecular and cellular biochemistry ·Vol. 40 ·No. 3 ·1981-11-13 ·Pages 173-87

Busch H, Goldknopf IL

Abstract

The data available at present indicates there are three distinct functions of ubiquitin, two of which are related to protein conjugation. The first of these has been extensively studied by our laboratory and others interested in nucleosomes and changes in chromatin states. The ubiquitin-histone (Ub-2A, Ub-2B) conjugation reaction now appears to be a very dynamic process. In the deconjugation (lyase) reaction, both the histone 2A and the ubiquitin are left intact and in a form which makes possible ready reconjugation. Accordingly, this may be a mechanism for "moment-to-moment" Control of the genome. The second function in which ubiquitin is conjugated involves proteolytic activity. This activity is correlated with protein turnover. In this process, the ubiquitin-protein conjugate apparently serves as a "signal" for the protease cleavage of the protein. The released ubiquitin is also intact and is probably available for reconjugation. In the third function, ubiquitin was suggested to serve as a "hormone". The studies thus far have been carried out primarily on induction of T- and B-lymphocytes, reduction or delay of Coombs' positivity and reduction of spleen weight. The precise physiological role of this reported function is still unclear, particularly because the ubiquitin used was probably not the physiologically active form.

MeSH Terms
Amino Acid Sequence Animals Chromatin/metabolism Chromosomal Proteins, Non-Histone/isolation & purification,metabolism Histones/metabolism Liver/metabolism Liver Regeneration Macromolecular Substances Nucleosomes/metabolism Peptide Fragments/analysis Rats Ubiquitins
Chemicals
Chromatin Chromosomal Proteins, Non-Histone Histones Macromolecular Substances Nucleosomes Peptide Fragments Ubiquitins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Busch H
Goldknopf I L
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Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00224611
Published
1981-11-13
Pages
173-87
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
Grants
NCI NIH HHS · CA-10893, P11 · United States
External Links
PubMed source DOI: 10.1007/BF00224611 View journal details
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