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PMID: 287057 Published · ppublish English Journal Article

Histone 1 is proximal to histone 2A and to A24.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 5 ·1979-05-00 ·Pages 2190-4

Bonner WM, Stedman JD

Abstract

Water-soluble carbodiimide crosslinks histones 1A and 1B to histone 2A and to semi-histone A24 in chromatin from mouse cells. The identities of the histone species present in the crosslinked dimers were determined by fingerprinting. The molar ratio of H1--A24 to H2A is the same as the molar ratio of A24 to H2A in these cells. The H1-H2A crosslinks form equally well in whole nuclei, lysed nuclei, and H1-containing mononucleosomes isolated from a sucrose gradient. These results suggest that there exist major H1 interactions within the nucleosome.

MeSH Terms
Animals Binding Sites Carbodiimides Cell Nucleus/ultrastructure Chickens Chromatin/ultrastructure Chromosomal Proteins, Non-Histone/metabolism DNA/metabolism Erythrocytes/ultrastructure Histones/metabolism Leukemia L1210 Mice Protein Binding Protein Conformation
Chemicals
Carbodiimides Chromatin Chromosomal Proteins, Non-Histone Histones DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bonner W M
Stedman J D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.5.2190
Published
1979-05-00
Pages
2190-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383563
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.5.2190 PMC Full Text View journal details
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