Home LiteratureArticle Details
PMID: 49060 Published · ppublish English Journal Article

A p60 polypeptide in the feline leukemia virus pseudotype of Moloney sarcoma virus with murine leukemia virus p30 antigenic determinants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2380-4

Oskarsson MK, Robey WG, Harris CL, Fischinger PJ, Haapala DK, Vande Woude GF

Abstract

A 60,000-dalton polypeptide (p60) has been identified in the feline leukemia virus (FeLV) pseudotype of Moloney sarcoma virus [MSV(FeLV)]. This polypeptide is present in the purified virus complex in concentrations greater than either the murine p30 or the feline p27. Purified p60 crossreacts immunologically with murine p30 group antiserum and contains several interspecies determinants, whereas the group specific determinant of FeLV p27 is not detected. Comparison of peptide fingerprints of p60 and murine p30 show many peptides in common. Limited digestion of p60 with either trypsin or chymotrypsin produced p30-35 and p20 peptides which retain the MuLV p30 group and interspecies antigenic activities. The p30 produced by both enzymes comigrates in polyacrylamide gels with the murine p30 of MSV(FeLV), thus suggesting that p60 may be an uncleaved precursor to p30.

MeSH Terms
Chromatography, Gel Chymotrypsin Cross Reactions Electrophoresis, Polyacrylamide Gel Epitopes Guanidines Immunodiffusion Leukemia Virus, Feline/analysis,immunology Leukemia Virus, Murine/immunology Molecular Weight Moloney murine leukemia virus/analysis,immunology Peptides/analysis,isolation & purification Trypsin
Chemicals
Epitopes Guanidines Peptides Chymotrypsin Trypsin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Oskarsson M K
Robey W G
Harris C L
Fischinger P J
Haapala D K
Vande Woude G F
References (25)
25 references, click to expand
  1. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  2. Aberrant viruses in cells infected with murine sarcoma virus-feline leukemia virus.
    J Natl Cancer Inst. 1975 Mar;54(3):763-66 PMID: 164569
  3. Large-volume purification of tumor viruses by use of zonal centrifuges.
    Appl Microbiol. 1972 May;23(5):1010-4 PMID: 4113253
  4. Revertants of mouse cells transformed by murine sarcoma virus. I. Characterization of flat and transformed sublines without a rescuable murine sarcoma virus.
    Virology. 1972 Oct;50(1):51-64 PMID: 4117128
  5. RNA in mammalian sarcoma virus transformed nonproducer cells homologous to murine leukemia virus RNA.
    Virology. 1973 Feb;51(2):370-82 PMID: 4120907
  6. Isolation of RD-114-like oncornavirus from a cat cell line.
    J Virol. 1973 Jun;11(6):978-85 PMID: 4123287
  7. Studies on the nucleic acid sequences of Kirsten sarcoma virus: a model for formation of a mammalian RNA-containing sarcoma virus.
    J Virol. 1973 Sep;12(3):458-63 PMID: 4127029
  8. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein.
    J Virol. 1974 Jan;13(1):171-80 PMID: 4129838
  9. Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells.
    Virology. 1969 May;38(1):174-9 PMID: 4306523
  10. An evaluation of film detection methods for weak beta-emitters, particularly tritium.
    Anal Biochem. 1970 Mar;34:188-205 PMID: 4314971
  11. Number and molecular weights of foot-and-mouth disease virus capsid proteins and the effects of maleylation.
    J Virol. 1971 Feb;7(2):250-9 PMID: 4329399
  12. Properties of mouse leukemia viruses. I. Characterization of various antisera and serological identification of viral components.
    Virology. 1972 Jan;47(1):197-209 PMID: 4333567
  13. Detection of some murine leukemia virus antigens in virus particles derived from 3T3 cells transformed only by murine sarcoma virus.
    Virology. 1972 Jan;47(1):229-35 PMID: 4333569
  14. Comparison of murine sarcoma viruses in nonproducer and S + L - -transformed cells.
    J Virol. 1972 Apr;9(4):701-4 PMID: 4336564
  15. Isolation of human amnion cells transformed by rescuable murine sarcoma virus.
    Nat New Biol. 1973 Mar 28;242(117):98-101 PMID: 4350532
  16. Identification of a large polypeptide precursor of avian oncornavirus proteins.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1734-8 PMID: 4352653
  17. Isolation of proteins by gel filtration in 6M guanidinium chloride: application to RNA tumor viruses.
    Anal Biochem. 1974 Jan;57(1):108-17 PMID: 4361621
  18. Simple, quantitative assay for both xenotropic murine leukemia and ecotropic feline leukemia viruses.
    J Virol. 1974 Jul;14(1):177-9 PMID: 4365331
  19. Murine type-C virus group-specific antigens: interstrain immunochemical, biophysical, and amino acid sequence differences.
    J Virol. 1974 Dec;14(6):1559-74 PMID: 4372411
  20. Polypeptides of mammalian oncornaviruses. II Characterization of murine leukemia virus polypeptide (p 15) bearing interspecies reactivity.
    Virology. 1975 Jan;63(1):48-59 PMID: 46125
  21. Structural aberrations in T-even bacteriophage. V. Effects of canavanine on the maturation and utilization of specific gene products.
    J Virol. 1974 Jun;13(6):1378-91 PMID: 4833614
  22. Proteins of the murine C-type RNA tumour viruses: isolation of a group-specific antigen by isoelectric focusing.
    J Gen Virol. 1970 Jul;8(1):1-10 PMID: 4990526
  23. A transmissible virus-induced lymphocytic leukemia of the cat.
    J Natl Cancer Inst. 1969 Jun;42(6):987-1014 PMID: 5793196
  24. Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses.
    Cancer Res. 1967 Sep;27(9):1672-7 PMID: 6057678
  25. Biosynthesis of Rauscher leukemia viral proteins.
    Cell. 1975 Jan;4(1):31-6 PMID: 803875
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2380
Published
1975-06-00
Pages
2380-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432762
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2380 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2