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PMID: 271971 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Presence of protein A24 in rat liver nucleosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5492-5

Goldknopf IL, French MF, Musso R, Busch H

Abstract

Two-dimensional gel profiles of the 0.2 M H2SO4-soluble proteins of monomer nucleosomal fractions were found to contain protein A24. Protein A24 is of interest because it is composed of histone 2A and "ubiquitin", apparently joined by an isopeptide linkage [Goldknopf, I.L. & Busch, H. (1977) Proc. Natl. Acad. Sci. USA 74, 864-868; Hunt, L.T. & Dayhoff, M.O. (1977) Biochem. Biophys. Res. Commun. 74, 650-655]. Monomer nucleosomal fractions were obtained by sucrose density gradient centrifugation of micrococcal nuclease digests of rat liver nuclei. As shown by their DNA size, the monomer fractions were highly purified. Proteins A24 and Bu, another protein of unknown characteristics, were found along with histones 1, 2A, 2B, 3, and 4 in the monomer fractions in relative amounts similar to those found in extracts from whole nuclei and chromatin. Other acid-soluble proteins found in the nuclear and chromatin extracts were essentially absent from the monomer fraction. Inasmuch as protein A24 and Bu were found in lesser amounts than the histones, it is suggested that they are associated with specialized subsets of nucleosomes.

MeSH Terms
Animals Chromatin/analysis,ultrastructure Chromosomal Proteins, Non-Histone/analysis Histones/analysis Liver/ultrastructure Micrococcal Nuclease/metabolism Rats
Chemicals
Chromatin Chromosomal Proteins, Non-Histone Histones Micrococcal Nuclease
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Goldknopf I L
French M F
Musso R
Busch H
References (37)
37 references, click to expand
  1. Determination of the nucleotide sequence of part of the regulatory region for the galactose operon from Escherichia coli.
    J Biol Chem. 1977 May 25;252(10):3538-47 PMID: 193835
  2. Higher order coiling of DNA in chromatin.
    Cell. 1977 Sep;12(1):83-100 PMID: 409504
  3. Structure of chromatin.
    Annu Rev Biochem. 1977;46:931-54 PMID: 332067
  4. Nucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):106-10 PMID: 319453
  5. Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):864-8 PMID: 265581
  6. The presence of high mobility group non-histone chromatin proteins in isolated nucleosomes.
    FEBS Lett. 1977 Jan 15;73(1):85-8 PMID: 838051
  7. Amino-terminal sequence identity of ubiquitin and the nonhistone component of nuclear protein A24.
    Biochem Biophys Res Commun. 1977 Jan 24;74(2):650-5 PMID: 836318
  8. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70 PMID: 1069279
  9. Low-angle neutron scattering from chromatin subunit particles.
    Nucleic Acids Res. 1975 Nov;2(11):2163-76 PMID: 1052536
  10. Identification of nonhistone chromatin proteins in chromatin subunits.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3458-62 PMID: 1068458
  11. Chromatin subunit small angle neutron scattering: a DNA rich coil surrounds a protein-DNA core.
    Biochem Biophys Res Commun. 1976 Sep 7;72(1):176-83 PMID: 985465
  12. The NH2- and COOH-terminal amino acid sequence of nuclear protein A24.
    J Biol Chem. 1976 Oct 10;251(19):5901-3 PMID: 972147
  13. Ribosomal genes of Xenopus laevis: evidence of nucleosomes in transcriptionally active chromatin.
    Science. 1976 Oct 29;194(4264):529-32 PMID: 973136
  14. Chromosomal proteins and chromatin structure.
    Annu Rev Biochem. 1975;44:725-74 PMID: 1094921
  15. Chemical probes of chromatin structure.
    Biochemistry. 1974 Aug 13;13(17):3622-8 PMID: 4367431
  16. Analysis of DNA of isolated chromatin subunits.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3978-82 PMID: 1060078
  17. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  18. Two-dimensional polyacrylamide gel electrophoresis of acid extractable nuclear proteins of normal rat liver and Novikoff hepatoma ascites cells.
    Biochem Biophys Res Commun. 1973 Apr 16;51(4):956-66 PMID: 4349999
  19. Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1316-20 PMID: 4351171
  20. Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):11-5 PMID: 1078892
  21. The complete amino acid sequence of ubiquitin, an adenylate cyclase stimulating polypeptide probably universal in living cells.
    Biochemistry. 1975 May 20;14(10):2214-8 PMID: 1170880
  22. Amino acid sequence of the center of the arginine-lysine-rich histone from calf thymus. The total sequence.
    J Biol Chem. 1972 Oct 10;247(19):6018-23 PMID: 4568599
  23. Amino acid sequence of the COOH-terminal portion of the arginine-lysine-rich histone of calf thymus.
    J Biol Chem. 1972 Jun 10;247(11):3589-91 PMID: 4555425
  24. Differences and similarities in chromatin structure of Neurospora crassa and higher eucaryotes.
    Cell. 1976 Jul;8(3):349-55 PMID: 133762
  25. Interactions of histone LAK (f2a2) with histones KAS (f2b) and GRK (f2a1).
    Biochemistry. 1974 May 7;13(10):2098-104 PMID: 4857060
  26. The effect of trypsin on nuclease-resistant chromatin fragments.
    J Biol Chem. 1974 Jan 10;249(1):152-6 PMID: 4855624
  27. Chromatin structure: a repeating unit of histones and DNA.
    Science. 1974 May 24;184(4139):868-71 PMID: 4825889
  28. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
    Biochem Biophys Res Commun. 1973 May 15;52(2):504-10 PMID: 4711166
  29. Presence of messenger specifying sequences in the DNA of chromatin subunits.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1572-5 PMID: 1064026
  30. Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes.
    Biochemistry. 1976 Jul 27;15(15):3307-14 PMID: 952859
  31. Quaternary structure of chromatin.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1043-5 PMID: 1055363
  32. Chromatin model calculations: Arrays of spherical nu bodies.
    Nucleic Acids Res. 1976 Jan;3(1):89-100 PMID: 1250708
  33. Remarkable similarities of peptide fingerprints of histone 2A and nonhistone chromosomal protein A24.
    Biochem Biophys Res Commun. 1975 Aug 4;65(3):951-60 PMID: 1156427
  34. Isolation and characterization of protein A24, a "histone-like" non-histone chromosomal protein.
    J Biol Chem. 1975 Sep 25;250(18):7182-7 PMID: 1165239
  35. The subunit structure of the eukaryotic chromosome.
    Nature. 1975 Jan 24;253(5489):245-9 PMID: 1167623
  36. Chromatin fragments resembling v bodies.
    Science. 1975 Jan 17;187(4172):173-5 PMID: 1111096
  37. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.
    Cell. 1975 Apr;4(4):281-300 PMID: 1122558
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5492
Published
1977-12-00
Pages
5492-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431777
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.12.5492 PMC Full Text View journal details
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