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PMID: 337304 Published · ppublish English Journal Article

Isopeptide linkage between N-alpha-monomethylalanine and lysine in ribosomal protein S11 from Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 11 ·1977-11-00 ·Pages 4905-8

Chen R, Chen-Schmeisser U

Abstract

Protein S11 from the Escherichia coli ribosome has a unique NH2-terminal structure not previously observed among ribosomal proteins. Owing to the formation of an isopeptide bond between a secondary amino acid (N-alpha-monomethylalanine) and the epsilon-amino group of the NH2-terminal lysine residue, a "branching point" is formed. Therefore, two amino acids are seen when the NH2 terminus of the protein is determined.

MeSH Terms
Alanine/analogs & derivatives,analysis Amino Acid Sequence Bacterial Proteins Chemical Phenomena Chemistry Escherichia coli/analysis Hydrolysis Leucyl Aminopeptidase Lysine/analysis Oligopeptides/chemical synthesis Ribosomal Proteins Trypsin
Chemicals
Bacterial Proteins Oligopeptides Ribosomal Proteins Leucyl Aminopeptidase Trypsin Lysine Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chen R
Chen-Schmeisser U
References (19)
19 references, click to expand
  1. THE STRUCTURE OF A CHYMOTRYPTIC PEPTIDE FROM PSEUDOMONAS CYTOCHROME C-551.
    Biochem J. 1963 Nov;89:379-80 PMID: 14084623
  2. The primary structure of protein L16 located at the peptidyltransferase center of Escherichia coli ribosomes.
    FEBS Lett. 1976 Sep 15;68(1):105-9 PMID: 786730
  3. Sequence determination of protein S9 from the Escherichia coli ribosome.
    Hoppe Seylers Z Physiol Chem. 1977 Nov;358(11):1415-30 PMID: 336507
  4. Occurrence of methylated amino acids as N-termini of proteins from Escherichia coli ribosomes.
    J Mol Biol. 1977 Apr;111(2):173-81 PMID: 323502
  5. Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):864-8 PMID: 265581
  6. Primary structure of protein L33 from the large subunit of the Escherichia coli ribosome.
    FEBS Lett. 1976 Sep 15;68(1):115-8 PMID: 786732
  7. The identification of isopeptide crosslinks in insoluble fibrin.
    Biochem Biophys Res Commun. 1968 Feb 26;30(4):356-62 PMID: 5637041
  8. Alteration in the acetylation level of ribosomal protein L12 during growth cycle of Escherichia coli.
    Proc Natl Acad Sci U S A. 1974 May;71(5):2136-40 PMID: 4600787
  9. 50-S ribosomal proteins. Peptide studies on two acidic proteins, A 1 and A 2 , isolated from 50-S ribosomes of Escherichia coli.
    Eur J Biochem. 1972 Jan 31;25(1):13-9 PMID: 4554103
  10. [Cross-link in fibrin polymerized by factor 13: epsilon-(gamma-glutamyl)lysine].
    Science. 1968 May 24;160(3830):892-3 PMID: 4967475
  11. Structure and function of ribosomes and their molecular components.
    Cold Spring Harb Symp Quant Biol. 1969;34:49-61 PMID: 4909519
  12. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
    Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449
  13. The sequence determination of a protein in a micro scale: the sequence analysis of ribosomal protein L34 of Escherichia coli.
    Hoppe Seylers Z Physiol Chem. 1976 Jun;357(6):873-86 PMID: 783033
  14. Primary structure of protein S18 from the small Escherichia coli ribosomal subunit.
    FEBS Lett. 1975 Nov 15;59(2):217-20 PMID: 776663
  15. Isolation of peptides containing citrulline and the cross-link, epsilon-(gamma-glutamyl)lysine, from hair medulla protein.
    Biochim Biophys Acta. 1976 Mar 18;427(1):315-24 PMID: 1260005
  16. Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac.
    Biochemistry. 1971 Dec 21;10(26):4912-21 PMID: 5134536
  17. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  18. [Improved synthesis of etert-butyloxycarbonyl-amino acids through pH stable reactions].
    Justus Liebigs Ann Chem. 1967;702:188-96 PMID: 5633713
  19. A protein sequenator.
    Eur J Biochem. 1967 Mar;1(1):80-91 PMID: 6059350
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.11.4905
Published
1977-11-00
Pages
4905-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432065
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.11.4905 PMC Full Text View journal details
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