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PMID: 2177590 Published · ppublish English Journal Article

Characterization of a plasmid involved with cointegrate formation and lactose metabolism in Lactococcus lactis subsp. lactis OZS1.

Archives of microbiology ·Vol. 154 ·No. 6 ·1990-00-00 ·Pages 560-5

Smigielski AJ

Abstract

A 55 kilobase (kb) plasmid (pOZS550) in the non-clumping Lactococcus lactis subsp. lactis strain OZS1 carrying genes for lactose metabolism was characterised. A mobilizable cointegrate plasmid which is formed between pOZS550 and pOZS448 carries the necessary information for conjugation and transfer. Cointegrate formation was found to involve an insertional element located on pOZS550. The insertion sequence was found to be identical to ISS1 located on pSK08 in the clumping L. lactis subsp. lactis strain ML3. Restriction maps of pOZS550 and pSK08 were similar suggesting a close ancestral relationship, although pSK08, in addition to the lactose metabolism genes, expressed genes for proteinase activity and cell clumping, which were not expressed by pOZS550, and carried two copies of ISS1 compared to one on pOZS550. Furthermore, hybridization of the 18 base pair inverted repeat, of the insertion sequence, with various L. lactis subsp. lactis strains and two L. lactis subsp. cremoris strains showed moderate to strong hybridization to one plasmid in each organism.

Related Genes
Lac Prt Rec
MeSH Terms
Base Sequence Blotting, Southern Cloning, Molecular Conjugation, Genetic DNA Transposable Elements DNA, Bacterial/chemistry Gene Expression Regulation, Bacterial Lactococcus lactis/genetics Lactose/genetics,metabolism Molecular Sequence Data Nucleic Acid Hybridization Plasmids Repetitive Sequences, Nucleic Acid Restriction Mapping
Chemicals
DNA Transposable Elements DNA, Bacterial Lactose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Smigielski A J
Department of Nutrition and Food Sciences, Utah State University.
References (16)
16 references, click to expand
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00248837
Published
1990-00-00
Pages
560-5
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00248837 View journal details
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