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PMID: 217882 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Calcium-binding modulator protein from the unfertilized egg of the sea urchin Arbacia punctulata.

The Journal of cell biology ·Vol. 80 ·No. 1 ·1979-01-00 ·Pages 211-8

Head JF, Mader S, Kaminer B

Abstract

We have purified and partly characterized a calcium-binding protein from the unfertilized egg of the sea urchin Arbacia punctulata. This protein closely resembles the calcium-binding modulator protein of bovine brain in its molecular weight, electrophoretic mobility, amino acid analysis, and peptide map. It activates bovine brain phosphodiesterase in the presence of calcium but has no effect on the phosphodiesterase of the Arbacia egg. Densitometric scanning of acrylamide gels of arbacia egg homogenates shows the modulator protein to represent 0.1% of the total protein of the egg. At 10(-4) M free calcium, the protein binds four calcium ions per 17,000-dalton molecule. We have used a column of rabbit skeletal muscle troponin-I covalently coupled to Sepharose 4B as an affinity column to selectively purify the Arbacia egg calcium-binding protein. This column has also been used to purify bovine brain modulator protein and may prove of general use in isolating similar proteins from other sources. The technique may be particularly helpful when only small quantities of starting material are available.

MeSH Terms

3',5'-Cyclic-AMP Phosphodiesterases/metabolism Amino Acids/analysis Animals Calmodulin/analysis,isolation & purification,pharmacology Carrier Proteins/analysis,isolation & purification Female Molecular Weight Ovum/analysis Sea Urchins

Chemicals

Amino Acids Calmodulin Carrier Proteins 3',5'-Cyclic-AMP Phosphodiesterases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Head J F

Mader S

Kaminer B

References (23)

23 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.80.1.211

Published

1979-01-00

Pages

211-8

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2110296

Subset

External Links

PubMed source DOI: 10.1083/jcb.80.1.211 PMC Full Text View journal details

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Calcium-binding modulator protein from the unfertilized egg of the sea urchin Arbacia punctulata.

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