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PMID: 218196 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Involvement of spectrin in membrane fusion: induction of fusion in human erythrocyte ghosts by proteolytic enzymes and its inhibition by antispectrin antibody.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 1 ·1979-01-00 ·Pages 318-22

Lalazar A, Loyter A

Abstract

In contrast to intact human erythrocytes, human erythrocyte ghosts can be agglutinated but not fused by Sendai virus. Membrane fusion can, however, be induced in virus-agglutinated erythrocyte ghosts by addition of proteolytic enzymes such as trypsin, papain, or Pronase. When erythrocyte ghosts were reacted with antispectrin antiserum, the antiserum inhibited both the induction of fusion and the proteolysis of the membrane spectrin. The correlation between the membrane fusion process and the membrane cytoskeleton is discussed.

MeSH Terms
Actins/metabolism Cell Fusion Erythrocyte Membrane/physiology Erythrocytes/physiology Humans Membrane Proteins/metabolism,physiology Parainfluenza Virus 1, Human Peptide Hydrolases/metabolism Spectrin/antagonists & inhibitors,immunology,physiology
Chemicals
Actins Membrane Proteins Spectrin Peptide Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lalazar A
Loyter A
References (22)
22 references, click to expand
  1. Reconstitution of intramembrane particles in recombinants of erythrocyte protein band 3 and lipid: effects of spectrin-actin association.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3891-5 PMID: 1069273
  2. Membrane fusion during secretion. A hypothesis based on electron microscope observation of Phytophthora Palmivora zoospores during encystment.
    J Cell Biol. 1977 Apr;73(1):161-81 PMID: 856830
  3. Participation of spectrin in Sendai virus-induced fusion of human erythrocyte ghosts.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1740-4 PMID: 205869
  4. Restoration of the fusion capacity of human erythrocyte ghosts by SH blocking reagents.
    Exp Cell Res. 1977 Jun;107(1):79-88 PMID: 193708
  5. Modification of cell membranes with viral envelopes during fusion of cells with HVJ (Sendai virus). III. Effects of mono- and di-saccharides on cell fusion and membrane movement of fused cells.
    Exp Cell Res. 1977 Aug;108(1):95-106 PMID: 196874
  6. Membrane ultrastructural changes during calcium phosphate-induced fusion of human erythrocyte ghosts.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2417-21 PMID: 329283
  7. Molecular events during membrane fusion. A study of exocytosis in rat peritoneal mast cells.
    J Cell Biol. 1977 Feb;72(2):242-59 PMID: 319098
  8. Thermotropic lateral translational motion of intramembrane particles in the inner mitochondrial membrane and its inhibition by artificial peripheral proteins.
    J Cell Biol. 1977 Feb;72(2):278-91 PMID: 833199
  9. Effects of incorporated trypsin on anion exchange and membrane proteins in human red blood cell ghosts.
    Biochim Biophys Acta. 1976 Dec 2;455(2):353-70 PMID: 999920
  10. Anionic sites of human erythrocyte membranes. I. Effects of trypsin, phospholipase C, and pH on the topography of bound positively charged colloidal particles.
    J Cell Biol. 1973 May;57(2):373-87 PMID: 4121289
  11. Trypsin action on the growth of Sendai virus in tissue culture cells. 3. Structural difference of Sendai viruses grown in eggs and tissue culture cells.
    J Virol. 1973 Dec;12(6):1457-65 PMID: 4357516
  12. Fusion of intact human erythrocytes and erythrocyte ghosts.
    J Cell Biol. 1974 Oct;63(1):1-11 PMID: 4371393
  13. Anionic sites of human erythrocyte membranes. II. Antispectrin-induced transmembrane aggregation of the binding sites for positively charged colloidal particles.
    J Cell Biol. 1973 Nov;59(2 Pt 1):395-406 PMID: 4141707
  14. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  15. Effect of specific antibodies on biological functions of the envelope components of Newcastle disease virus.
    Virology. 1974 Oct;61(2):354-60 PMID: 4213724
  16. Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus).
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):4071-5 PMID: 172893
  17. Isolation of spectrin from erythrocyte membranes.
    Methods Enzymol. 1974;32:275-7 PMID: 4216739
  18. Intramembrane particle aggregation in erythrocyte ghosts. I. The effects of protein removal.
    J Cell Biol. 1974 Dec;63(3):1018-36 PMID: 4215819
  19. Role of sulfhydryl groups in erythrocyte membrane structure.
    Biochemistry. 1973 Jan 2;12(1):171-6 PMID: 4683480
  20. Promotion of cell fusion by divalent cation ionophores.
    Nature. 1975 Jul 17;256(5514):208-9 PMID: 1097941
  21. The transmembrane proteins in the plasma membrane of normal human erythrocytes. Evaluation employing lactoperoxidase and proteases.
    Biochemistry. 1975 Dec 16;14(25):5512-6 PMID: 1201276
  22. Non-parallel transport of membrane proteins and content proteins during assembly of the secretory granule in rat parotid gland.
    Biochim Biophys Acta. 1975 Jan 14;375(1):87-105 PMID: 1111576
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.1.318
Published
1979-01-00
Pages
318-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC382930
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.1.318 PMC Full Text View journal details
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