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PMID: 2184337 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Antibodies to recombinant HIV-1 vif, tat, and nef proteins in human sera.

Medical microbiology and immunology ·Vol. 179 ·No. 1 ·1990-00-00 ·Pages 1-11

Wieland U, Kühn JE, Jassoy C, Rübsamen-Waigmann H, Wolber V, Braun RW

Abstract

The prevalence of antibodies against HIV-1 regulatory proteins in sera of HIV-infected patients from different stages of disease was investigated. HIV-1 vif, tat, and nef genes were cloned in procaryotic vectors and were expressed as MS-2 fusion proteins (vif and nef) or as a non-fusion protein (tat). These recombinant proteins were employed in immunoblot experiments. The specificity of the recognition was confirmed by competition experiments and with control sera from HIV-negative patients. Analysis of 136 serum samples revealed a high percentage of antibodies against nef, irrespective of the stage of disease. Antibodies against tat were found less frequently and increased from 16% to 40% with disease progression. Vif antibodies were detected only in a low percentage in early stages of disease, but their prevalence increased to 36% and 72% with progression of disease to AIDS-related complex and AIDS. Our data suggest that the detection of antibodies against nef may represent an additional and useful marker for the diagnosis of HIV infection, whereas the detection of vif antibodies may indicate disease progression.

MeSH Terms

Blotting, Western Cloning, Molecular Electrophoresis, Polyacrylamide Gel Gene Products, nef/genetics,immunology Gene Products, tat/genetics,immunology Gene Products, vif HIV Antibodies/analysis HIV Infections/diagnosis HIV Seroprevalence HIV-1/genetics,immunology Humans Plasmids Recombinant Proteins/immunology Trans-Activators/genetics Viral Regulatory and Accessory Proteins/genetics,immunology nef Gene Products, Human Immunodeficiency Virus tat Gene Products, Human Immunodeficiency Virus vif Gene Products, Human Immunodeficiency Virus

Chemicals

Gene Products, nef Gene Products, tat Gene Products, vif HIV Antibodies Recombinant Proteins Trans-Activators Viral Regulatory and Accessory Proteins nef Gene Products, Human Immunodeficiency Virus tat Gene Products, Human Immunodeficiency Virus vif Gene Products, Human Immunodeficiency Virus

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Wieland U

Institut für Medizinische Virologie, Universität Heidelberg, Federal Republic of Germany.

Kühn J E

Jassoy C

Rübsamen-Waigmann H

Wolber V

Braun R W

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Article Info

Journal

Medical microbiology and immunology

Abbr.

Med Microbiol Immunol

ISSN

0300-8584

DOI

10.1007/BF00190145

Published

1990-00-00

Pages

1-11

Language

English

Region

Germany

NLM ID

0314524

Subset

External Links

PubMed source DOI: 10.1007/BF00190145 View journal details

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Antibodies to recombinant HIV-1 vif, tat, and nef proteins in human sera.

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