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PMID: 2185215 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential mRNA stability controls relative gene expression within the plasmid-encoded arsenical resistance operon.

Journal of bacteriology ·Vol. 172 ·No. 5 ·1990-05-00 ·Pages 2367-71

Owolabi JB, Rosen BP

Abstract

The arsenical resistance (ars) operon of the conjugative plasmid R773 encodes an ATP-driven anion extrusion pump, conferring bacterial resistance to arsenicals. The operon contains a regulatory gene, arsR, and three structural genes, arsA, arsB, and arsC. The hydrophilic ArsA and ArsC proteins are produced in large amounts, but the hydrophobic ArsB protein, an integral membrane polypeptide, is synthesized in limited quantities. Northern (RNA-DNA) hybridizations provide evidence that the inducible operon is regulated at the level of transcription. The genes were transcribed in the presence of an inducer (arsenite) as a single polycistronic mRNA with an approximate size of 4.4 kilobases (kb). This transcript was processed to generate relatively stable mRNA species: one of 2.7 kb, encoding the ArsR and ArsA proteins, and a second of 0.5 kb, encoding the ArsC protein. Segmental differences in stability within the polycistronic transcript are proposed to account for the differential expression of the ars genes. In addition, analysis of the mRNA structure at the 5' end of arsB suggests a potential translational block to the synthesis of this membrane protein.

MeSH Terms
Arsenicals/pharmacology Base Sequence Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics Gene Expression Regulation, Bacterial Genotype Kinetics Molecular Sequence Data Nucleic Acid Conformation Operon R Factors RNA, Messenger/genetics Transcription, Genetic
Chemicals
Arsenicals RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Owolabi J B
Department of Biochemistry, School of Medicine, Wayne State University, Detroit, Michigan 48201.
Rosen B P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.5.2367-2371.1990
Published
1990-05-00
Pages
2367-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208871
Subset
IM
Grants
NIAID NIH HHS · AI19793 · United States
External Links
PubMed source DOI: 10.1128/jb.172.5.2367-2371.1990 PMC Full Text View journal details
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