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PMID: 218557 Published · ppublish English Journal Article

Calcium ions and the regulation of NAD+-linked isocitrate dehydrogenase from the mitochondria of rat heart and other tissues.

The Biochemical journal ·Vol. 176 ·No. 3 ·1978-12-15 ·Pages 899-906

Denton RM, Richards DA, Chin JG

Abstract

The effects of Ca2+ on the activity of isocitrate dehydrogenase (NAD+) in extracts of rat heart mitochondria were explored in the presence of MgCl2 by using EGTA buffers. In the absence of ADP, Ca2+ (about 30 micrometer) resulted in a slight increase in apparent Km for threo-Ds-isocitrate; in the presence of ADP, Ca2+ (about 25 micrometer) greatly lowered the apparent Km for threo-Ds-isocitrate from 227 micrometer to 53 micrometer without changing the maximum velocity. At 100 micrometer-threo-Ds-isocitrate and 1 mM-ADP, there was an 8-fold activation by Ca2+, with a Km for Ca2+ of 1.2 micrometer. This activation was also observed with Sr2+ (Km 3.1 micrometer), but not with Mn2+ (at concentrations below 2.5 micrometer). Similar effects of Ca2+ were also observed on isocitrate dehydrogenase (NAD+) activity in extracts of mitochondria from liver, kidney, brown adipose tissue and white adipose tissue of the rat. The possible regulatory role of changes in the intramitochondrial concentration of Ca2+ is discussed.

MeSH Terms
Animals Calcium/pharmacology Cations, Divalent Egtazic Acid/pharmacology Female In Vitro Techniques Isocitrate Dehydrogenase/metabolism Kinetics Male Mitochondria/metabolism Mitochondria, Heart/drug effects,enzymology NAD/metabolism Rats
Chemicals
Cations, Divalent NAD Egtazic Acid Isocitrate Dehydrogenase Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Denton R M
Richards D A
Chin J G
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28 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1760899
Published
1978-12-15
Pages
899-906
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186314
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1760899 PMC Full Text View journal details
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