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PMID: 2196175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of trans-activating proteins in the generation of active chromatin at the PHO5 promoter in S. cerevisiae.

The EMBO journal ·Vol. 9 ·No. 8 ·1990-08-00 ·Pages 2523-8

Fascher KD, Schmitz J, Hörz W

Abstract

Induction of the PHO5 gene in Saccharomyces cerevisiae by phosphate starvation was previously shown to be accompanied by the removal of four positioned nucleosomes from the promoter. We have now investigated the role of two trans-activating proteins, encoded by PHO2 and PHO4, which bind to the PHO5 promoter. Both proteins are absolutely required for the chromatin transition to occur as shown by analysis of null mutants of the two genes. Transformation of these mutant strains with plasmids containing the respective genes restores the wild type chromatin response. Increasing the gene dosage of PHO2 and of PHO4 makes it possible to differentiate functionally between the two proteins. From over-expressing PHO4 in a wild type and also in a pho2 null mutant strain and complementary experiments with PHO2, it is concluded that the PHO4 protein is the primary trigger for the chromatin transition, consistent with one of its two binding sites being located between positioned nucleosomes in repressed chromatin and thereby accessible. PHO2, the binding site of which is located within a nucleosome under conditions of PHO5 repression, contributes to the chromatin transition either by destabilizing histone-DNA interactions or by under-going interactions with PHO4.

MeSH Terms
Acid Phosphatase/genetics,metabolism Chromatin/metabolism DNA-Binding Proteins Fungal Proteins/genetics,metabolism Gene Expression Homeodomain Proteins Mutation Plasmids Promoter Regions, Genetic Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors
Chemicals
Chromatin DNA-Binding Proteins Fungal Proteins Homeodomain Proteins PHO2 protein, S cerevisiae PHO4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Acid Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fascher K D
Institut für Physiologische Chemie, Universität München, FRG.
Schmitz J
Hörz W
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32 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-08-00
Pages
2523-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552282
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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