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PMID: 3536481 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements.

The EMBO journal ·Vol. 5 ·No. 10 ·1986-10-00 ·Pages 2689-96

Almer A, Rudolph H, Hinnen A, Hörz W

Abstract

The chromatin fine structure in the promoter region of PHO5, the structural gene for a strongly regulated acid phosphatase in yeast, was analyzed. An upstream activating sequence 367 bp away from the start of the coding sequence that is essential for gene induction was found to reside in the center of a hypersensitive region under conditions of PHO5 repression. Under these conditions three related elements at positions -469, -245 and -185 are contained within precisely positioned nucleosomes located on both sides of the hypersensitive region. Upon PHO5 induction the chromatin structure of the promoter undergoes a defined transition, in the course of which two nucleosomes upstream and two nucleosomes downstream of the hypersensitive site are selectively removed. In this way approximately 600 bp upstream of the PHO5 coding sequence become highly accessible and all four elements are free to interact with putative regulatory proteins. These findings suggest a mechanism by which the chromatin structure participates in the functioning of a regulated promoter.

MeSH Terms
Acid Phosphatase/biosynthesis,genetics Base Sequence Chromatin/ultrastructure Enzyme Induction Genes Genes, Fungal Nucleosomes/ultrastructure Promoter Regions, Genetic Saccharomyces cerevisiae/enzymology,genetics
Chemicals
Chromatin Nucleosomes Acid Phosphatase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Almer A
Rudolph H
Hinnen A
Hörz W
References (29)
29 references, click to expand
  1. Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult beta-globin gene: nature of the binding domain.
    Cell. 1985 May;41(1):21-30 PMID: 2986843
  2. Organization of the GAL1-GAL10 intergenic control region chromatin.
    Nucleic Acids Res. 1984 Nov 26;12(22):8457-74 PMID: 6095201
  3. Assembly and propagation of repressed and depressed chromosomal states.
    Cell. 1985 Oct;42(3):705-11 PMID: 2996773
  4. One-step gene replacement in yeast by cotransformation.
    Gene. 1985;36(1-2):87-95 PMID: 2998940
  5. Nuclease hypersensitive regions with adjacent positioned nucleosomes mark the gene boundaries of the PHO5/PHO3 locus in yeast.
    EMBO J. 1986 Oct;5(10):2681-7 PMID: 3023055
  6. Structure of the transcriptionally repressed phosphate-repressible acid phosphatase gene (PHO5) of Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jan;6(1):38-46 PMID: 3537687
  7. DNAase I-hypersensitive sites of chromatin.
    Cell. 1981 Dec;27(3 Pt 2):413-5 PMID: 6101195
  8. Specific DNA binding of GAL4, a positive regulatory protein of yeast.
    Cell. 1985 Apr;40(4):767-74 PMID: 3886158
  9. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
    Nature. 1980 Aug 28;286(5776):854-60 PMID: 6774262
  10. Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae.
    J Cell Biol. 1980 Jul;86(1):123-8 PMID: 6998984
  11. Structure of transcribing chromatin.
    Prog Nucleic Acid Res Mol Biol. 1980;24:1-55 PMID: 6256821
  12. Sequence specific cleavage of DNA by micrococcal nuclease.
    Nucleic Acids Res. 1981 Jun 25;9(12):2643-58 PMID: 7279658
  13. A 200 base pair region at the 5' end of the chicken adult beta-globin gene is accessible to nuclease digestion.
    Cell. 1981 Nov;27(1 Pt 2):45-55 PMID: 6276024
  14. Chromatin conformational changes accompany transcriptional activation of a glucose-repressed gene in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):253-6 PMID: 7043456
  15. Chromatin.
    Annu Rev Biochem. 1982;51:89-121 PMID: 6287925
  16. A complex of interacting DNAase I-hypersensitive sites near the Drosophila glue protein gene, Sgs4.
    Cell. 1982 Jun;29(2):601-7 PMID: 6214314
  17. Chromatin structure and gene activity: the role of nonhistone chromosomal proteins.
    CRC Crit Rev Biochem. 1982;13(1):1-86 PMID: 6751690
  18. Modulation of chromatin structure associated with derepression of the acid phosphatase gene of Saccharomyces cerevisiae.
    J Biol Chem. 1983 Jun 10;258(11):7223-7 PMID: 6304084
  19. Promoter elements, regulatory elements, and chromatin structure of the yeast his3 gene.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:901-10 PMID: 6305590
  20. Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):95-9 PMID: 6320177
  21. Yeast promoters: positive and negative elements.
    Cell. 1984 Apr;36(4):799-800 PMID: 6368004
  22. The locus of sequence-directed and protein-induced DNA bending.
    Nature. 1984 Apr 5-11;308(5959):509-13 PMID: 6323997
  23. Anatomy of hypersensitive sites.
    Nature. 1984 May 17-23;309(5965):213-4 PMID: 6325942
  24. Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.
    Nature. 1984 May 17-23;309(5965):229-34 PMID: 6325944
  25. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  26. Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences.
    EMBO J. 1982;1(6):675-80 PMID: 6329697
  27. Alternative sets of DNase I-hypersensitive sites characterize the various functional states of the chicken lysozyme gene.
    Nature. 1984 Sep 13-19;311(5982):163-5 PMID: 6236374
  28. Structural analysis of the two tandemly repeated acid phosphatase genes in yeast.
    Nucleic Acids Res. 1984 Oct 25;12(20):7721-39 PMID: 6093051
  29. DNase I-hypersensitive sites in the galactose gene cluster of Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Jun;5(6):1522-4 PMID: 3897838
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-10-00
Pages
2689-96
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167170
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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