Home LiteratureArticle Details
PMID: 7043456 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chromatin conformational changes accompany transcriptional activation of a glucose-repressed gene in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 2 ·1982-01-00 ·Pages 253-6

Sledziewski A, Young ET

Abstract

In the present study we have analyzed the kinetics of DNase I digestion of the two alcohol dehydrogenase (ADH) genes within yeast nuclei. We have found that in yeast grown on glucose the constitutively transcribed ADCI gene is much more sensitive to DNase I digestion than is the repressible ADR2 gene. In yeast grown on ethanol, both genes are transcribed and both exhibit the same sensitivity to DNase I attack. We have also found and mapped DNase I hypersensitive sites near the 5' ends of constitutive and repressible ADH genes. These sites are well correlated with the position at which transcription is initiated.

MeSH Terms
Alcohol Oxidoreductases/genetics Chromatin/ultrastructure Deoxyribonucleases/metabolism Enzyme Repression Gene Expression Regulation Kinetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Chromatin Alcohol Oxidoreductases Deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sledziewski A
Young E T
References (24)
24 references, click to expand
  1. Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae. II. Two loci controlling synthesis of the glucose-repressible ADH II.
    Mol Gen Genet. 1975;138(2):157-64 PMID: 1105150
  2. Cis-dominant regulatory mutations affecting the formation of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae.
    Mol Gen Genet. 1976 Jun 15;145(3):327-33 PMID: 781520
  3. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  4. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70 PMID: 1069279
  5. An altered subunit configuration associated with the actively transcribed DNA of integrated adenovirus genes.
    Cell. 1977 Nov;12(3):783-94 PMID: 562719
  6. Genes transcribed at diverse rates have a similar conformation in chromatin.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4867-71 PMID: 270719
  7. Transcriptional role of yeast deoxyribonucleic acid dependent ribonucleic acid polymerase III.
    Biochemistry. 1978 Feb 21;17(4):750-8 PMID: 341977
  8. Active conformation of the globin genes in uninduced and induced mouse erythroleukemia cells.
    Cell. 1978 Jul;14(3):511-21 PMID: 99241
  9. Regulation of expression and chromosomal subunit conformation of avian retrovirus genomes.
    Cell. 1978 Aug;14(4):865-78 PMID: 210959
  10. Sites in simian virus 40 chromatin which are preferentially cleaved by endonucleases.
    Cell. 1978 Dec;15(4):1511-8 PMID: 215331
  11. A stretch of "late" SV40 viral DNA about 400 bp long which includes the origin of replication is specifically exposed in SV40 minichromosomes.
    Cell. 1979 Feb;16(2):453-66 PMID: 222461
  12. The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity.
    Cell. 1979 Apr;16(4):807-14 PMID: 455450
  13. Yeast chromatin is uniformly digested by DNase-I.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4285-8 PMID: 388438
  14. Isolation of the structural gene for alcohol dehydrogenase by genetic complementation in yeast.
    Nature. 1980 Jan 10;283(5743):214-6 PMID: 6985717
  15. Isolation and characterization of further cis- and trans-acting regulatory elements involved in the synthesis of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae.
    Mol Gen Genet. 1979 Nov;176(3):427-31 PMID: 392242
  16. Hb switching in chickens.
    Cell. 1980 Apr;19(4):973-80 PMID: 6155217
  17. Tissue-specific DNA cleavages in the globin chromatin domain introduced by DNAase I.
    Cell. 1980 Jun;20(2):451-60 PMID: 7388947
  18. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
    Nature. 1980 Aug 28;286(5776):854-60 PMID: 6774262
  19. A simple, rapid, and sensitive DNA assay procedure.
    Anal Biochem. 1980 Mar 1;102(2):344-52 PMID: 6158890
  20. Chromatin structure of the histone genes of D. melanogaster.
    Cell. 1981 Feb;23(2):401-9 PMID: 6258802
  21. Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II.
    Cell. 1981 Feb;23(2):605-14 PMID: 6258806
  22. Tissue-specific exposure of chromatin structure at the 5' terminus of the rat preproinsulin II gene.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1577-80 PMID: 6262815
  23. DNase I hypersensitive sites in Drosophila chromatin occur at the 5' ends of regions of transcription.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):143-6 PMID: 6264428
  24. A positive regulatory gene is required for accumulation of the functional messenger RNA for the glucose-repressible alcohol dehydrogenase from Saccharomyces cerevisiae.
    J Mol Biol. 1981 Jun 5;148(4):355-68 PMID: 7031263
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.2.253
Published
1982-01-00
Pages
253-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345704
Subset
IM
Grants
NIGMS NIH HHS · GM 26079 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.2.253 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2