Home LiteratureArticle Details
PMID: 6093051 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural analysis of the two tandemly repeated acid phosphatase genes in yeast.

Nucleic acids research ·Vol. 12 ·No. 20 ·1984-10-25 ·Pages 7721-39

Bajwa W, Meyhack B, Rudolph H, Schweingruber AM, Hinnen A

Abstract

We have sequenced the genetically linked genes for repressible (PHO5) and and constitutive (PHO3) acid phosphatase from S. cerevisiae. Both genes are located on a 3.91 Kb BamHI and HpaI fragment, in the order (5') PHO5, PHO3 (3'). The mRNA transcripts have been analysed by S1-nuclease mapping. They show heterogenous initiation sites. Each of the PHO5 and PHO3 genes codes for 467 amino acids as deduced from the DNA sequence. The coding regions of the two genes show homology both at the nucleotide (82%) and the amino acid (87%) level. In the coding sequences, long stretches of homologous regions are flanked by small non-homologous regions. The nucleotide homology (65%) extends to some length into the 5' and 3' non-coding flanking sequences. Further upstream sequences are unrelated. The comparison of the NH2-terminal amino acid sequence deduced from the nucleotide sequence, with that of purified repressible acid phosphatase revealed the presence of a putative signal peptide.

MeSH Terms
Acid Phosphatase/genetics Amino Acid Sequence Base Sequence DNA Restriction Enzymes Genes Genes, Fungal Genetic Linkage Plasmids Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/enzymology,genetics
Chemicals
DNA Restriction Enzymes Acid Phosphatase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bajwa W
Meyhack B
Rudolph H
Schweingruber A M
Hinnen A
References (45)
45 references, click to expand
  1. Genes coding for the structure of the acid phosphatases in Saccharomyces cerevisiae.
    Mol Gen Genet. 1975 Dec 30;143(1):65-70 PMID: 765744
  2. Size of primary transcripts in Ehrlich ascites cells as measured by tetraphosphate determination.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1994-8 PMID: 1064868
  3. Sequence analysis of eukaryotic mRNA.
    Prog Nucleic Acid Res Mol Biol. 1976;19:123-34 PMID: 828277
  4. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  7. The primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiae.
    J Biol Chem. 1979 Oct 10;254(19):9839-45 PMID: 385592
  8. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  9. Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1980 Mar 25;255(6):2596-605 PMID: 6244283
  10. Isolation and sequence of the gene for iso-2-cytochrome c in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):541-5 PMID: 6244566
  11. In vitro synthesis of repressible yeast acid phosphatase: identification of multiple mRNAs and products.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4504-8 PMID: 7001459
  12. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.
    Cell. 1980 Oct;21(3):627-38 PMID: 7438203
  13. The primary structures of two yeast enolase genes. Homology between the 5' noncoding flanking regions of yeast enolase and glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1981 Feb 10;256(3):1385-95 PMID: 6256394
  14. A position effect in the control of transcription at yeast mating type loci.
    Nature. 1981 Jan 22;289(5795):244-50 PMID: 6256656
  15. Isolation of yeast genes with mRNA levels controlled by phosphate concentration.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6541-5 PMID: 6256743
  16. Histone H2B genes of yeast encode two different proteins.
    Cell. 1980 Dec;22(3):799-805 PMID: 7006833
  17. Structure and function of the PHO82-pho4 locus controlling the synthesis of repressible acid phosphatase of Saccharomyces cerevisiae.
    J Bacteriol. 1981 Jan;145(1):221-32 PMID: 7007314
  18. Homology and concerted evolution at the alpha 1 and alpha 2 loci of human alpha-globin.
    Nature. 1981 Mar 5;290(5801):26-9 PMID: 7010180
  19. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  20. Molecular evidence for genetic exchanges among ribosomal genes on nonhomologous chromosomes in man and apes.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7323-7 PMID: 6261251
  21. Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2258-62 PMID: 6264471
  22. Comparison of mouse immunoglobulin gamma 2a and gamma 2b chain genes suggests that exons can be exchanged between genes in a multigenic family.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2442-6 PMID: 6787604
  23. Gene conversion: some implications for immunoglobulin genes.
    Cell. 1981 Jun;24(3):592-4 PMID: 7018693
  24. N-Glycosylation of yeast proteins. Characterization of the solubilized oligosaccharyl transferase.
    Eur J Biochem. 1981 May;116(1):101-8 PMID: 7018901
  25. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  26. Order of events in the yeast secretory pathway.
    Cell. 1981 Aug;25(2):461-9 PMID: 7026045
  27. Multiple differences between the nucleic acid sequences of the IgG2aa and IgG2ab alleles of the mouse.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4495-9 PMID: 6170065
  28. Human nucleolus organizers on nonhomologous chromosomes can share the same ribosomal gene variants.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5744-8 PMID: 6272316
  29. The cyc1-11 mutation in yeast reverts by recombination with a nonallelic gene: composite genes determining the iso-cytochromes c.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6334-8 PMID: 6273865
  30. The actin gene in yeast Saccharomyces cerevisiae: 5' and 3' end mapping, flanking and putative regulatory sequences.
    Nucleic Acids Res. 1981 Dec 11;9(23):6339-50 PMID: 6275358
  31. Transcription of the his3 gene region in Saccharomyces cerevisiae.
    J Mol Biol. 1981 Nov 5;152(3):535-52 PMID: 6173489
  32. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
    J Biol Chem. 1982 Mar 25;257(6):3018-25 PMID: 6277922
  33. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  34. The role of core-oligosaccharide in formation of an active acid phosphatase and its secretion by yeast protoplasts.
    J Biochem. 1982 Jan;91(1):191-200 PMID: 6802810
  35. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  36. Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1982 Apr 24;10(8):2625-37 PMID: 6281737
  37. Purification and identification of inactive forms of repressible and constitutive acid phosphatase in yeast.
    Biochim Biophys Acta. 1982 Aug 6;717(2):203-9 PMID: 6810953
  38. Nucleotide sequence of the yeast alcohol dehydrogenase II gene.
    J Biol Chem. 1983 Feb 25;258(4):2674-82 PMID: 6337160
  39. The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide.
    Nucleic Acids Res. 1983 Mar 25;11(6):1657-72 PMID: 6300772
  40. RNA and homology mapping of two DNA fragments with repressible acid phosphatase genes from Saccharomyces cerevisiae.
    Mol Cell Biol. 1983 Apr;3(4):562-9 PMID: 6343839
  41. Comparative analysis of the 5'-end regions of two repressible acid phosphatase genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1983 Apr;3(4):570-9 PMID: 6343840
  42. Sequence determination of eglin C using combined microtechniques of amino acid analysis, peptide isolation, and automatic Edman degradation.
    Anal Biochem. 1983 Apr 1;130(1):65-71 PMID: 6869810
  43. Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences.
    EMBO J. 1982;1(6):675-80 PMID: 6329697
  44. Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae.
    J Bacteriol. 1973 Feb;113(2):727-38 PMID: 4570606
  45. Biosynthesis of acid phosphatase of baker's yeast. Characterization of a protoplast-bound fraction containing precursors of the exo-enzyme.
    Biochim Biophys Acta. 1975 Feb 19;377(2):331-42 PMID: 235303
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.20.7721
Published
1984-10-25
Pages
7721-39
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320196
Subset
IM
Databases
GENBANK
M13473, M13474, X01079
External Links
PubMed source DOI: 10.1093/nar/12.20.7721 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2