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PMID: 6320177 Published · ppublish English Journal Article

Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 1 ·1984-01-00 ·Pages 95-9

Emerson BM, Felsenfeld G

Abstract

Chromatin in the 5' flanking region of the transcriptionally active chicken adult beta(beta A) globin gene is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the beta A-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.

MeSH Terms
Animals Cell Nucleus/metabolism Cellulose/analogs & derivatives Chickens Chromatography, Affinity DNA/analogs & derivatives,genetics DNA Restriction Enzymes Deoxyribonucleases/metabolism Genes Globins/genetics
Chemicals
DNA-cellulose Globins Cellulose DNA Deoxyribonucleases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Emerson B M
Felsenfeld G
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.1.95
Published
1984-01-00
Pages
95-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344617
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.1.95 PMC Full Text View journal details
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