Home LiteratureArticle Details
PMID: 2196430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Initiation of meiosis and sporulation in Saccharomyces cerevisiae requires a novel protein kinase homologue.

Molecular & general genetics : MGG ·Vol. 221 ·No. 2 ·1990-04-00 ·Pages 176-86

Yoshida M, Kawaguchi H, Sakata Y, Kominami K, Hirano M, Shima H, Akada R, Yamashita I

Abstract

SME1 was cloned due to its high copy number effect: it enabled MATa/MAT alpha diploid cells to undergo meiosis and sporulation in a vegetative medium. Disruption of SME1 resulted in a recessive Spo- phenotype. These results suggest that SME1 is a positive regulator for meiosis. DNA sequencing analysis revealed an open reading frame of 645 amino acids. An amino terminal peptide of ca 400 amino acids in the deduced protein was similar to known protein kinases. Transcription of SME1 was regulated negatively by nitrogen and glucose and positively by MATa/MAT alpha and IME1, another positive regulator gene of meiosis. By complementation analysis, SME1 was found to be identical to IME2, which had been shown to be important in meiosis. These results suggest that IME1 product stimulates meiosis by activating transcription of SME1 (IME2) and that protein phosphorylation is required for initiation of meiosis.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle Proteins Cloning, Molecular Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Genes, Fungal Intracellular Signaling Peptides and Proteins Meiosis Molecular Sequence Data Protein Kinases/genetics,metabolism Protein Serine-Threonine Kinases Restriction Mapping Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Spores, Fungal Transcription, Genetic
Chemicals
Cell Cycle Proteins Fungal Proteins Intracellular Signaling Peptides and Proteins Saccharomyces cerevisiae Proteins Protein Kinases IME2 protein, S cerevisiae Protein Serine-Threonine Kinases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Yoshida M
Center for Gene Science, Hiroshima University, Higashi-Hiroshima, Japan.
Kawaguchi H
Sakata Y
Kominami K
Hirano M
Shima H
Akada R
Yamashita I
References (30)
30 references, click to expand
  1. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  2. Isolation and sequencing of cDNA clones encoding alpha and beta subunits of Drosophila melanogaster casein kinase II.
    Mol Cell Biol. 1987 Oct;7(10):3409-17 PMID: 3119988
  3. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  4. Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
    Nature. 1987 May 7-13;327(6117):31-5 PMID: 3553962
  5. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
    Methods Enzymol. 1983;100:293-308 PMID: 6312261
  6. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  7. Initiation of meiosis in yeast mutants defective in adenylate cyclase and cyclic AMP-dependent protein kinase.
    Cell. 1983 Feb;32(2):417-23 PMID: 6297794
  8. Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
    J Biol Chem. 1977 Mar 10;252(5):1712-8 PMID: 190238
  9. Structure and sequence of the centromeric DNA of chromosome 4 in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jan;6(1):241-5 PMID: 3537685
  10. Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1794-8 PMID: 3470758
  11. Gene fusion is a possible mechanism underlying the evolution of STA1.
    J Bacteriol. 1987 May;169(5):2142-9 PMID: 3106330
  12. Corrections to the nucleotide sequence of the src gene of Rous sarcoma virus.
    Nature. 1983 Feb 24;301(5902):736-8 PMID: 6298633
  13. The major promoter element of rRNA transcription in yeast lies 2 kb upstream.
    Cell. 1984 Dec;39(3 Pt 2):663-73 PMID: 6096018
  14. Activation of meiosis and sporulation by repression of the RME1 product in yeast.
    Nature. 1986 Feb 27-Mar 5;319(6056):738-42 PMID: 3513021
  15. Isolation and characterization of Caenorhabditis elegans DNA sequences homologous to the v-abl oncogene.
    Proc Natl Acad Sci U S A. 1986 Apr;83(7):2172-6 PMID: 3457381
  16. Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase.
    Cell. 1987 Jul 17;50(2):277-87 PMID: 3036373
  17. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  18. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  19. Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes.
    Nature. 1984 Jan 12-18;307(5947):183-5 PMID: 6361575
  20. Identification of protein kinases by computer.
    Nature. 1988 May 26;333(6171):308 PMID: 3374567
  21. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  22. In yeast, RAS proteins are controlling elements of adenylate cyclase.
    Cell. 1985 Jan;40(1):27-36 PMID: 2981630
  23. A specific inhibitor of the ran1+ protein kinase regulates entry into meiosis in Schizosaccharomyces pombe.
    Nature. 1988 Apr 7;332(6164):509-14 PMID: 3357510
  24. IME1, a positive regulator gene of meiosis in S. cerevisiae.
    Cell. 1988 Mar 25;52(6):853-62 PMID: 3280136
  25. In vivo ligation of linear DNA molecules to circular forms in the yeast Saccharomyces cerevisiae.
    J Bacteriol. 1983 Aug;155(2):747-54 PMID: 6307979
  26. A transcriptional cascade governs entry into meiosis in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 May;9(5):2142-52 PMID: 2664470
  27. Polymorphic extracellular glucoamylase genes and their evolutionary origin in the yeast Saccharomyces diastaticus.
    J Bacteriol. 1985 Feb;161(2):574-82 PMID: 3918018
  28. Regulation of mating and meiosis in yeast by the mating-type region.
    Genetics. 1976 Feb;82(2):187-206 PMID: 770230
  29. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
    Gene. 1979 Dec;8(1):121-33 PMID: 395029
  30. A gene which encodes a predicted protein kinase can restore some functions of the ras gene in fission yeast.
    EMBO J. 1988 Apr;7(4):985-93 PMID: 3042386
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00261718
Published
1990-04-00
Pages
176-86
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00261718
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2