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PMID: 21969552 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Natural gas and temperature structured a microbial community response to the Deepwater Horizon oil spill.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 109 ·No. 50 ·2012-12-11 ·Pages 20292-7

Redmond MC, Valentine DL

Abstract

Microbial communities present in the Gulf of Mexico rapidly responded to the Deepwater Horizon oil spill. In deep water plumes, these communities were initially dominated by members of Oceanospirillales, Colwellia, and Cycloclasticus. None of these groups were abundant in surface oil slick samples, and Colwellia was much more abundant in oil-degrading enrichment cultures incubated at 4 °C than at room temperature, suggesting that the colder temperatures at plume depth favored the development of these communities. These groups decreased in abundance after the well was capped in July, but the addition of hydrocarbons in laboratory incubations of deep waters from the Gulf of Mexico stimulated Colwellia's growth. Colwellia was the primary organism that incorporated (13)C from ethane and propane in stable isotope probing experiments, and given its abundance in environmental samples at the time that ethane and propane oxidation rates were high, it is likely that Colwellia was active in ethane and propane oxidation in situ. Colwellia also incorporated (13)C benzene, and Colwellia's abundance in crude oil enrichments without natural gas suggests that it has the ability to consume a wide range of hydrocarbon compounds or their degradation products. However, the fact that ethane and propane alone were capable of stimulating the growth of Colwellia, and to a lesser extent, Oceanospirillales, suggests that high natural gas content of this spill may have provided an advantage to these organisms.

MeSH Terms
Bacteria/drug effects,genetics,metabolism Ecosystem Gulf of Mexico Molecular Sequence Data Natural Gas/analysis,toxicity Petroleum Pollution/adverse effects Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Temperature Water Microbiology Water Pollutants, Chemical/analysis,metabolism,toxicity
Chemicals
Natural Gas RNA, Bacterial RNA, Ribosomal, 16S Water Pollutants, Chemical
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Redmond Molly C
Department of Earth Science and Marine Science Institute, University of California, Santa Barbara, CA 93106, USA.
Valentine David L
References (38)
38 references, click to expand
  1. Novel methylotrophic bacteria isolated from the River Thames (London, UK).
    Environ Microbiol. 2008 Dec;10(12):3225-36 PMID: 18681896
  2. The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses.
    Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10913-8 PMID: 16043709
  3. Bellerophon: a program to detect chimeric sequences in multiple sequence alignments.
    Bioinformatics. 2004 Sep 22;20(14):2317-9 PMID: 15073015
  4. Methanotrophic bacteria.
    Microbiol Rev. 1996 Jun;60(2):439-71 PMID: 8801441
  5. Culturability and In situ abundance of pelagic bacteria from the North Sea.
    Appl Environ Microbiol. 2000 Jul;66(7):3044-51 PMID: 10877804
  6. Responses of microbial communities in Arctic sea ice after contamination by crude petroleum oil.
    Microb Ecol. 2008 Apr;55(3):540-52 PMID: 17805918
  7. Tracking hydrocarbon plume transport and biodegradation at Deepwater Horizon.
    Science. 2010 Oct 8;330(6001):201-4 PMID: 20724584
  8. Biodegradability of dispersed crude oil at two different temperatures.
    Mar Pollut Bull. 2007 May;54(5):545-53 PMID: 17316707
  9. Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis.
    FEMS Microbiol Rev. 1997 Nov;21(3):213-29 PMID: 9451814
  10. Natural assemblages of marine proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter.
    Appl Environ Microbiol. 2000 Apr;66(4):1692-7 PMID: 10742262
  11. Adaptations to energy stress dictate the ecology and evolution of the Archaea.
    Nat Rev Microbiol. 2007 Apr;5(4):316-23 PMID: 17334387
  12. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities.
    Appl Environ Microbiol. 2009 Dec;75(23):7537-41 PMID: 19801464
  13. Matching phylogeny and metabolism in the uncultured marine bacteria, one cell at a time.
    Proc Natl Acad Sci U S A. 2007 May 22;104(21):9052-7 PMID: 17502618
  14. Archaea in coastal marine environments.
    Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5685-9 PMID: 1608980
  15. Effects of temperature and biostimulation on oil-degrading microbial communities in temperate estuarine waters.
    Environ Microbiol. 2007 Jan;9(1):177-86 PMID: 17227422
  16. Dynamics of the hydrocarbon-degrading Cycloclasticus bacteria during mesocosm-simulated oil spills.
    Environ Microbiol. 2007 Oct;9(10):2551-62 PMID: 17803779
  17. Methane assimilation and trophic interactions with marine Methylomicrobium in deep-water coral reef sediment off the coast of Norway.
    FEMS Microbiol Ecol. 2008 Nov;66(2):320-30 PMID: 18811651
  18. Deep-sea oil plume enriches indigenous oil-degrading bacteria.
    Science. 2010 Oct 8;330(6001):204-8 PMID: 20736401
  19. Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov.
    Appl Environ Microbiol. 1999 Jan;65(1):251-9 PMID: 9872786
  20. Genetic diversity and potential function of microbial symbionts associated with newly discovered species of Osedax polychaete worms.
    Appl Environ Microbiol. 2007 Apr;73(7):2314-23 PMID: 17277220
  21. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy.
    Appl Environ Microbiol. 2007 Aug;73(16):5261-7 PMID: 17586664
  22. Degradation of alkanes by bacteria.
    Environ Microbiol. 2009 Oct;11(10):2477-90 PMID: 19807712
  23. Identification of novel methane-, ethane-, and propane-oxidizing bacteria at marine hydrocarbon seeps by stable isotope probing.
    Appl Environ Microbiol. 2010 Oct;76(19):6412-22 PMID: 20675448
  24. Hydrography shapes bacterial biogeography of the deep Arctic Ocean.
    ISME J. 2010 Apr;4(4):564-76 PMID: 20010630
  25. Obligate oil-degrading marine bacteria.
    Curr Opin Biotechnol. 2007 Jun;18(3):257-66 PMID: 17493798
  26. Marine microorganisms make a meal of oil.
    Nat Rev Microbiol. 2006 Mar;4(3):173-82 PMID: 16489346
  27. Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization.
    Appl Environ Microbiol. 2000 Dec;66(12):5116-22 PMID: 11097877
  28. Composition and fate of gas and oil released to the water column during the Deepwater Horizon oil spill.
    Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20229-34 PMID: 21768331
  29. Dynamic autoinoculation and the microbial ecology of a deep water hydrocarbon irruption.
    Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20286-91 PMID: 22233808
  30. Oleispira antarctica gen. nov., sp. nov., a novel hydrocarbonoclastic marine bacterium isolated from Antarctic coastal sea water.
    Int J Syst Evol Microbiol. 2003 May;53(Pt 3):779-785 PMID: 12807200
  31. T-REX: software for the processing and analysis of T-RFLP data.
    BMC Bioinformatics. 2009 Jun 06;10:171 PMID: 19500385
  32. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
    Mol Biol Evol. 2007 Aug;24(8):1596-9 PMID: 17488738
  33. Response of Archaeal communities in beach sediments to spilled oil and bioremediation.
    Appl Environ Microbiol. 2004 May;70(5):2614-20 PMID: 15128510
  34. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
  35. DNA-, rRNA- and mRNA-based stable isotope probing of aerobic methanotrophs in lake sediment.
    Environ Microbiol. 2011 May;13(5):1153-67 PMID: 21261798
  36. A persistent oxygen anomaly reveals the fate of spilled methane in the deep Gulf of Mexico.
    Science. 2011 Jan 21;331(6015):312-5 PMID: 21212320
  37. At least 1 in 20 16S rRNA sequence records currently held in public repositories is estimated to contain substantial anomalies.
    Appl Environ Microbiol. 2005 Dec;71(12):7724-36 PMID: 16332745
  38. Propane respiration jump-starts microbial response to a deep oil spill.
    Science. 2010 Oct 8;330(6001):208-11 PMID: 20847236
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1108756108
Published
2012-12-11
Epub
2011-00-03
Pages
20292-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC3528494
Subset
IM
Databases
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PubMed source DOI: 10.1073/pnas.1108756108 PMC Full Text View journal details
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