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PMID: 2198269 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Intrinsic bends and integration host factor binding at F plasmid oriT.

Journal of bacteriology ·Vol. 172 ·No. 8 ·1990-08-00 ·Pages 4603-9

Tsai MM, Fu YH, Deonier RC

Abstract

F plasmid oriT DNA extending from the F kilobase coordinate 66.7 (base pair [bp] 1 on the oriT sequence map) rightward to bp 527 was analyzed for intrinsic bends (by permutation assays) and for binding of integration host factor (IHF) (by gel retardation and DNase footprinting). Intrinsic bending of the 527-bp fragment (bend center approximately at bp 240) was represented as a composite of at least two components located near bp 170 and near bp 260. IHF bound primarily to a site extending from bp 165 to 195 and with lower affinity to a site extending from bp 287 to 319. The intrinsic curvature and sequences to which IHF binds (IHF is known to bend DNA) may play a structural role in oriT function.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence Binding Sites DNA, Bacterial/genetics,metabolism DNA-Binding Proteins/metabolism Escherichia coli/genetics,metabolism F Factor Integration Host Factors Kinetics Mathematics Molecular Sequence Data Nucleic Acid Conformation Nucleotide Mapping Plasmids Restriction Mapping
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Integration Host Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tsai M M
Department of Biological Sciences, University of Southern California, Los Angeles 90089-1340.
Fu Y H
Deonier R C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.8.4603-4609.1990
Published
1990-08-00
Pages
4603-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213294
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.8.4603-4609.1990 PMC Full Text View journal details
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