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PMID: 3611030 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

oriT sequence of the antibiotic resistance plasmid R100.

Journal of bacteriology ·Vol. 169 ·No. 8 ·1987-08-00 ·Pages 3829-32

McIntire SA, Dempsey WB

Abstract

We present the nucleotide sequence of the oriT region from plasmid R100. Comparison to other IncF plasmids revealed homology around the proposed nick sites as well as conservation of inverted repeated sequences in the nonhomologous region. Three areas showed strong homology (eight of nine nucleotides) to the consensus sequence for binding of integration host factor, suggesting a role for this DNA-binding protein in nicking at oriT.

MeSH Terms
Anti-Bacterial Agents/pharmacology Conjugation, Genetic DNA, Bacterial/genetics,metabolism DNA-Binding Proteins/metabolism F Factor R Factors Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid
Chemicals
Anti-Bacterial Agents DNA, Bacterial DNA-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McIntire S A
Dempsey W B
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40 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.8.3829-3832.1987
Published
1987-08-00
Pages
3829-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212476
Subset
IM
Grants
NIAID NIH HHS · AI-17402 · United States
Databases
GENBANK
M17148
External Links
PubMed source DOI: 10.1128/jb.169.8.3829-3832.1987 PMC Full Text View journal details
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