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PMID: 6277869 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA homology of the promoter-distal regions of the tra operons of sex factors F and R100 in Escherichia coli K-12.

Journal of bacteriology ·Vol. 150 ·No. 1 ·1982-04-00 ·Pages 389-94

Manning PA, Morelli G

Abstract

The promoter-distal regions of the tra operons of F and R100-1 were analyzed by heteroduplex analysis, and the regions of nonhomology were identified. A common EcoRI restriction site was shown to be present, and this has allowed the physical maps to be aligned.

MeSH Terms

Base Sequence Conjugation, Genetic DNA Restriction Enzymes Deoxyribonuclease EcoRI Escherichia coli/genetics F Factor Nucleic Acid Heteroduplexes Operon Plasmids

Chemicals

Nucleic Acid Heteroduplexes DNA Restriction Enzymes Deoxyribonuclease EcoRI

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Manning P A

Morelli G

References (9)

9 references, click to expand

Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.
J Mol Biol. 1973 Apr 5;75(2):235-55 PMID: 4580676
Heteroduplex mapping of small plasmids derived from R-factor R12: in vivo recombination occurs at IS1 insertion sequences.
Gene. 1977;2(3-4):193-210 PMID: 344140
Assignment of tra cistrons to EcoRI fragments of F sex factor DNA.
J Bacteriol. 1978 Mar;133(3):1383-92 PMID: 346570
Tra cistrons and proteins encoded by the Escherichia coli antibiotic resistance plasmid R6-5.
Mol Gen Genet. 1978 Jul 11;163(2):169-79 PMID: 355846
Restriction endonuclease mapping of pSC101 and pMB9.
Mol Gen Genet. 1978 Sep 8;164(3):285-8 PMID: 714015
Construction and BamHL analysis of chimeric plasmids containing EcoRL DNA fragments of the F sex factor.
Plasmid. 1978 Feb;1(2):174-86 PMID: 372961
Lambda transducing phages derived from a FinO- R100::lambda cointegrate plasmid: proteins encoded by the R100 replication/incompatibility region and the antibiotic resistance determinant.
Mol Gen Genet. 1979 Nov;176(3):319-34 PMID: 160490
Analysis of the promoter-distal region of the tra operon of the F sex factor of Escherichia coli K-12 encoded by EcoRI restriction fragments f17, f19, and f2.
J Bacteriol. 1982 Apr;150(1):76-88 PMID: 6277873
Promoter-distal region of the tra operon of F-like sex factor R100 in Escherichia coli K-12.
J Bacteriol. 1982 Apr;150(1):89-99 PMID: 6277874

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.150.1.389-394.1982

Published

1982-04-00

Pages

389-94

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC220126

Subset

External Links

PubMed source DOI: 10.1128/jb.150.1.389-394.1982 PMC Full Text View journal details

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DNA homology of the promoter-distal regions of the tra operons of sex factors F and R100 in Escherichia coli K-12.

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