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PMID: 714015 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Restriction endonuclease mapping of pSC101 and pMB9.

Molecular & general genetics : MGG ·Vol. 164 ·No. 3 ·1978-09-08 ·Pages 285-8

Tait RC, Boyer HW

Abstract

A restriction endonuclease analysis of the plasmids pSC101 and pMB9 has allowed a determination of the alterations that occurred in the tetracycline resistance locus during the construction of pMB9 from pSC101. The genes for four of the polypeptides involved in tetracycline resistance have been positioned on the restriction endonuclease map of pSC101.

MeSH Terms
DNA Restriction Enzymes/metabolism DNA, Bacterial/isolation & purification Electrophoresis, Polyacrylamide Gel Genes Genetic Linkage R Factors Recombination, Genetic Tetracycline/pharmacology
Chemicals
DNA, Bacterial DNA Restriction Enzymes Tetracycline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tait R C
Boyer H W
References (9)
9 references, click to expand
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    Gene. 1977;2(2):75-93 PMID: 344136
  4. On the nature of tetracycline resistance controlled by the plasmid pSC101.
    Cell. 1978 Jan;13(1):73-81 PMID: 340050
  5. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons.
    Nature. 1976 Jan 29;259(5541):285-90 PMID: 765836
  6. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  7. Protein expression in E. coli minicells by recombinant plasmids.
    Cell. 1977 Mar;10(3):521-36 PMID: 403011
  8. Translation of Drosophila melanogaster sequences in Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):5041-5 PMID: 412196
  9. Origin of replication of pBR345 plasmid DNA.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5265-9 PMID: 271952
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333158
Published
1978-09-08
Pages
285-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00333158
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