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PMID: 346570 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Assignment of tra cistrons to EcoRI fragments of F sex factor DNA.

Journal of bacteriology ·Vol. 133 ·No. 3 ·1978-03-00 ·Pages 1383-92

Achtman M, Skurray RA, Thompson R, Helmuth R, Hall S, Beutin L, Clark AJ

Abstract

We describe here the cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector. These plasmids, as well as the series of Skurray et al. (Proc. Natl. Acad. Sci. U.S.A. 73:64-68, 1976), have been used to refine the map positions of tra cistrons on the F factor as well as to define a new DNA transfer cistron, traM. The current map of the tra cistrons is presented. None of the known tra cistrons, with the exception of traG, straddles an EcoRI site. The EcoRI site at 82 kilobases splits the traG cistron into two portions, an operator-proximal portion necessary for F pilus synthesis and an operator distal portion involved in conjugation itself. The operon structure of the tra cistrons was reevaluated, and we found that traI is at least partially independent of transcription of the traA to traD operon.

MeSH Terms
Conjugation, Genetic DNA Restriction Enzymes/metabolism DNA, Bacterial/genetics,metabolism Escherichia coli/genetics F Factor Genes Genetic Complementation Test Operon
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Achtman M
Skurray R A
Thompson R
Helmuth R
Hall S
Beutin L
Clark A J
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.133.3.1383-1392.1978
Published
1978-03-00
Pages
1383-92
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222176
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.133.3.1383-1392.1978 PMC Full Text View journal details
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