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PMID: 2198574 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Enhancement of RNA polymerase binding to promoters by a transcriptional activator, OmpR, in Escherichia coli: its positive and negative effects on transcription.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5940-4

Tsung K, Brissette RE, Inouye M

Abstract

The OmpR binding sequence (OBS) in the upstream region of the ompF promoter of Escherichia coli was fused to 27 synthetic promoters. Transcription from a number of weak promoters, regardless of their sequences, was dramatically activated in the presence of OmpR, a transcriptional activator. In vivo DNA footprinting revealed that OmpR enhanced the binding of RNA polymerase to the promoters. This enhancement was essential for transcription of weak promoters, while OmpR binding to the OBS fused to a strong promoter was inhibitory for transcription. These results indicate that OmpR stabilizes the formation of an RNA polymerase-promoter complex, possibly a closed promoter complex, and that a transcription activator can serve not only as a positive but also as a negative regulator for gene expression.

MeSH Terms
Bacterial Outer Membrane Proteins/metabolism Base Sequence DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology,genetics Molecular Sequence Data Nucleotide Mapping Oligonucleotide Probes/chemical synthesis Promoter Regions, Genetic Protein Binding Restriction Mapping Transcription, Genetic
Chemicals
Bacterial Outer Membrane Proteins Oligonucleotide Probes DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tsung K
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey at Rutgers, Piscataway 08854.
Brissette R E
Inouye M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5940
Published
1990-08-00
Pages
5940-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54445
Subset
IM
Grants
NIGMS NIH HHS · GM19043 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.15.5940 PMC Full Text View journal details
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