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PMID: 2199063 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The three postblastoderm cell cycles of Drosophila embryogenesis are regulated in G2 by string.

Cell ·Vol. 62 ·No. 3 ·1990-08-10 ·Pages 469-80

Edgar BA, O'Farrell PH

Abstract

The string (stg) locus of Drosophila encodes a factor that is thought to trigger mitosis by activating the p34cdc2 protein kinase. stg is required for mitosis early in development and is transcribed in a dynamic pattern that anticipates the pattern of embryonic cell divisions. Here we show that differential cell cycle regulation during postblastoderm development (cell cycles 14-16) occurs in G2. We demonstrate that stg mRNA expressed from a heat shock promotor triggers mitosis, and an associated S phase, in G2 cells during these cycles. Hence, differential cell cycle timing at this developmental stage is controlled by stg. Finally, we use heat-induced stg expression to alter the normal pattern of embryonic mitoses. Surprisingly, the complex mitotic pattern evident during normal development is not essential for many features of pattern formation or for viability.

MeSH Terms
Animals Blastoderm/cytology CDC2 Protein Kinase Cell Cycle Cell Division DNA Probes Drosophila/cytology,embryology,genetics Embryo, Nonmammalian/cytology,physiology Feedback Heat-Shock Proteins/genetics Hot Temperature Interphase Mitosis Nucleic Acid Hybridization Phosphoproteins/genetics Protein Kinases/genetics
Chemicals
DNA Probes Heat-Shock Proteins Phosphoproteins Protein Kinases CDC2 Protein Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Edgar B A
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
O'Farrell P H
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(90)90012-4
Published
1990-08-10
Pages
469-80
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2753418
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037193 · United States
NIGMS NIH HHS · R01 GM037193-10 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(90)90012-4 PMC Full Text View journal details
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