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PMID: 2139805 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The roles of Drosophila cyclins A and B in mitotic control.

Cell ·Vol. 61 ·No. 3 ·1990-05-04 ·Pages 535-47

Lehner CF, O'Farrell PH

Abstract

We have cloned, sequenced, and characterized the expression of a Drosophila cyclin B gene. The independent evolutionary conservation of A- and B-type cyclins implies that they have distinct roles. Indeed, in mutant embryos deficient in cyclin A, cells that accumulate only cyclin B do not enter mitosis. Thus, in vivo, cyclin B is not sufficient for mitosis. Furthermore, we find that the two cyclins are coexpressed in all proliferating cells throughout development. Though lacking a formal demonstration that cyclin B is essential as it is in other organisms, we propose that each of these proteins fulfills a distinct and essential role in the cell cycle.

MeSH Terms
Amino Acid Sequence Animals Antibodies Base Sequence Biological Evolution Cell Cycle Cloning, Molecular Cyclins Drosophila/cytology,embryology,genetics Embryo, Nonmammalian/cytology Gene Expression Genes Insect Hormones/genetics,isolation & purification,physiology Interphase Invertebrate Hormones/genetics,isolation & purification,physiology Mitosis Molecular Sequence Data Mutation Nucleic Acid Hybridization Sequence Homology, Nucleic Acid
Chemicals
Antibodies Cyclins Insect Hormones Invertebrate Hormones
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lehner C F
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
O'Farrell P H
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48 references, click to expand
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(90)90535-m
Published
1990-05-04
Pages
535-47
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2753431
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037193 · United States
NIGMS NIH HHS · R01 GM037193-10 · United States
Databases
GENBANK
M33192
External Links
PubMed source DOI: 10.1016/0092-8674(90)90535-m PMC Full Text View journal details
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