Home LiteratureArticle Details
PMID: 6087289 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence, structure, and codon preference of the Drosophila ribosomal protein 49 gene.

Nucleic acids research ·Vol. 12 ·No. 13 ·1984-07-11 ·Pages 5495-513

O'Connell PO, Rosbash M

Abstract

In this communication, we describe several features of the D. melanogaster gene which codes for ribosomal protein 49 (rp49). Nucleotide sequence analysis in conjunction with primer extension and S1 nuclease protection experiments show that the structure of the rp49 gene consists of a 102 bp 5' exon, a single 59 bp intron, and a 420 bp 3' exon, encoding a total of 132 amino acids. The rp49 gene shares many features with other abundantly expressed Drosophila genes, including codon preference, which are discussed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Codon DNA Restriction Enzymes Drosophila melanogaster/genetics Genes RNA, Messenger Ribosomal Proteins/genetics Transcription, Genetic
Chemicals
Codon RNA, Messenger Ribosomal Proteins ribosomal protein 49 DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
O'Connell P O
Rosbash M
References (45)
45 references, click to expand
  1. Promoter sequences of eukaryotic protein-coding genes.
    Science. 1980 Sep 19;209(4463):1406-14 PMID: 6251548
  2. Yeast use translational control to compensate for extra copies of a ribosomal protein gene.
    Cell. 1982 Jun;29(2):347-55 PMID: 7116444
  3. Transposition of cloned P elements into Drosophila germ line chromosomes.
    Science. 1982 Oct 22;218(4570):341-7 PMID: 6289435
  4. Genetic transformation of Drosophila with transposable element vectors.
    Science. 1982 Oct 22;218(4570):348-53 PMID: 6289436
  5. Expression of ribosomal-protein genes in Xenopus laevis development.
    Cell. 1982 Aug;30(1):163-71 PMID: 6889922
  6. Isolation of a cloned DNA segment containing a ribosomal protein gene of Drosophila melanogaster.
    Gene. 1982 Jun;18(3):267-76 PMID: 6127281
  7. Cuticle protein genes of Drosophila: structure, organization and evolution of four clustered genes.
    Cell. 1982 Jul;29(3):1027-40 PMID: 6817923
  8. Isolation and structural analysis of ribosomal protein genes in Xenopus laevis. Homology between sequences present in the gene and in several different messenger RNAs.
    J Mol Biol. 1982 Nov 5;161(3):353-71 PMID: 6296397
  9. Sequence and structure conservation in yolk proteins and their genes.
    J Mol Biol. 1983 Mar 15;164(4):481-92 PMID: 6405043
  10. Messenger RNA for ribosomal proteins in yeast.
    J Mol Biol. 1983 Mar 25;165(1):79-89 PMID: 6341608
  11. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  12. Regulation of ribosomal protein mRNA content and translation in growth-stimulated mouse fibroblasts.
    Mol Cell Biol. 1982 Jun;2(6):685-93 PMID: 14582163
  13. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.
    J Virol. 1974 Nov;14(5):1235-44 PMID: 4372397
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  16. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  17. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  18. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  19. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.
    Nucleic Acids Res. 1979 Aug 10;6(11):3559-67 PMID: 573889
  20. The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
    Cell. 1979 Oct;18(2):485-99 PMID: 498280
  21. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  22. Isolation of cloned DNA sequences containing ribosomal protein genes from Saccharomyces cerevisiae.
    Cell. 1979 Dec;18(4):1247-59 PMID: 391408
  23. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
  24. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  25. Isolation and mapping of a cloned ribosomal protein gene of Drosophila melanogaster.
    Nature. 1980 Jun 26;285(5767):674-6 PMID: 6771656
  26. Changes in regulation of ribosomal protein synthesis during vegetative growth and sporulation of Saccharomyces cerevisiae.
    J Bacteriol. 1980 Sep;143(3):1411-9 PMID: 6997272
  27. The 5' terminus of the precursor ribosomal RNA of Saccharomyces cerevisiae.
    Nucleic Acids Res. 1980 Jun 25;8(12):2679-89 PMID: 6159579
  28. Ubiquitous and gene-specific regulatory 5' sequences in a sea urchin histone DNA clone coding for histone protein variants.
    Nucleic Acids Res. 1980 Mar 11;8(5):957-77 PMID: 7443547
  29. Expression of ribosomal proteins during Drosophila early development.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5649-53 PMID: 6777772
  30. Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.
    Nucleic Acids Res. 1981 Mar 11;9(5):1069-86 PMID: 6112733
  31. Primary sequence of the 5' flanking regions of the Drosophila heat shock genes in chromosome subdivision 67B.
    Nucleic Acids Res. 1981 Apr 10;9(7):1627-42 PMID: 6262735
  32. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  33. Mammals have multiple genes for individual ribosomal proteins.
    Cell. 1981 May;24(2):301-6 PMID: 7237549
  34. Chromosomal distribution of ribosomal protein genes in the mouse.
    Cell. 1981 May;24(2):307-12 PMID: 7237550
  35. Sequence homologies in the 5' regions of four Drosophila heat-shock genes.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3775-8 PMID: 6791161
  36. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  37. The genes for fifteen ribosomal proteins of Saccharomyces cerevisiae.
    J Biol Chem. 1981 Oct 10;256(19):10176-83 PMID: 6268628
  38. Ribosomal protein mRNAs increase dramatically during Xenopus development.
    Dev Biol. 1981 Oct 30;87(2):330-9 PMID: 6169575
  39. Isolation of cloned ribosomal protein genes from the yeast Saccharomyces carlsbergensis.
    Gene. 1981 Sep;14(4):279-87 PMID: 6170538
  40. The sequence of the Drosophila melanogaster gene for yolk protein 1.
    Nucleic Acids Res. 1981 Dec 11;9(23):6407-19 PMID: 6275360
  41. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  42. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  43. Coordinate regulation of ribosomal protein mRNA level in regenerating rat liver. Study with the corresponding mouse cloned cDNAs.
    Nucleic Acids Res. 1982 Feb 11;10(3):789-801 PMID: 6121318
  44. DNase I hypersensitive sites of the chromatin for Drosophila melanogaster ribosomal protein 49 gene.
    Nucleic Acids Res. 1981 Dec 21;9(24):6749-62 PMID: 6278445
  45. A rapid enzymatic DNA sequencing technique: determination of sequence alterations in early simian virus 40 temperature sensitive and deletion mutants.
    Nucleic Acids Res. 1980 May 24;8(10):2225-40 PMID: 6253881
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.13.5495
Published
1984-07-11
Pages
5495-513
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318935
Subset
IM
Grants
NIGMS NIH HHS · GM23549 · United States
Databases
GENBANK
X00848
External Links
PubMed source DOI: 10.1093/nar/12.13.5495 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2