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PMID: 2460740 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alternative RNA splicing that is spatially regulated: generation of transcripts from the Antennapedia gene of Drosophila melanogaster with different protein-coding regions.

Molecular and cellular biology ·Vol. 8 ·No. 10 ·1988-10-00 ·Pages 4143-54

Stroeher VL, Gaiser JC, Garber RL

Abstract

We have shown previously that transcription of the Drosophila homeotic gene Antennapedia results in four major RNA species which differ in long 5'- and 3'-untranslated sequences. The protein-coding portion of these transcripts, however, is located in exons common to all. Using RNase protection assays and further cDNA clone isolation, we have now detected two alternative splicing events between exons of this region. These result in four RNA variations which, if translated, would encode a family of Antennapedia proteins. By analyzing transcripts from various developmental stages and isolated tissues, we show that alternative splicing is under strict temporal and spatial regulation. For example, while similar patterns of splicing were found for all wild-type thoracic imaginal disks examined, these differed distinctly from the patterns observed in neural tissues. Our results suggest that individual RNAs may be associated with different biological roles, and provide molecular evidence that the Antennapedia gene is involved in multiple functions.

MeSH Terms
Age Factors Amino Acid Sequence Animals DNA/genetics DNA Probes Drosophila melanogaster/embryology,genetics Exons Gene Expression Regulation Genes, Homeobox Molecular Sequence Data RNA RNA Splicing RNA, Antisense RNA, Messenger/genetics
Chemicals
DNA Probes RNA, Antisense RNA, Messenger RNA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stroeher V L
Department of Biological Structure, University of Washington, Seattle 98195.
Gaiser J C
Garber R L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.10.4143-4154.1988
Published
1988-10-00
Pages
4143-54
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365483
Subset
IM
Grants
NIGMS NIH HHS · GM33219 · United States
NICHD NIH HHS · T32 HD07183 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.10.4143-4154.1988 PMC Full Text View journal details
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