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PMID: 6172778 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.

Nucleic acids research ·Vol. 9 ·No. 23 ·1981-12-11 ·Pages 6351-68

Mount SM, Steitz JA

Abstract

U1 RNA from cultured Drosophila melanogaster cells (Kc) was identified by its ability to be recognized, as an RNP, by anti-(U1)RNP antibodies from human lupus patients. Its sequence was deduced largely from direct analysis of the RNA molecule and then confirmed by DNA sequence determinations on a genomic clone isolated by hybridization to Drosophila U1 RNA. The Drosophila U1 RNA sequence exhibits 72% agreement with human U1 RNA. Nucleotides 3-11, which are complementary to the entire consensus sequence for donor (5') splice junctions in hnRNA, and to part of the acceptor (3') consensus, are exactly conserved. However, nucleotides 14-21, postulated to interact only with acceptor junctions, differ. Comparison of the Drosophila U1 sequence with vertebrate U1 sequences allows a particular secondary structure model to be preferred over others. These results are consistent with the hypothesis that U1 snRNPs are involved in splicing, but suggest specific modifications of the model detailing molecular interactions between U1 RNA and hnRNA during the splicing reaction.

MeSH Terms
Animals Antibodies Autoimmune Diseases/immunology Base Sequence Cells, Cultured Cloning, Molecular Drosophila melanogaster/genetics Humans Lupus Erythematosus, Systemic/immunology Nucleic Acid Conformation Nucleic Acid Hybridization RNA/genetics,immunology RNA, Small Nuclear Ribonuclease T1 Ribonucleoproteins/immunology
Chemicals
Antibodies RNA, Small Nuclear Ribonucleoproteins RNA Ribonuclease T1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mount S M
Steitz J A
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.23.6351
Published
1981-12-11
Pages
6351-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327608
Subset
IM
Databases
GENBANK
J01156, K00787
External Links
PubMed source DOI: 10.1093/nar/9.23.6351 PMC Full Text View journal details
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