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PMID: 3658675 Published · ppublish English Comparative Study Journal Article

RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression.

Nucleic acids research ·Vol. 15 ·No. 17 ·1987-09-11 ·Pages 7155-74

Shapiro MB, Senapathy P

Abstract

A systematic analysis of the RNA splice junction sequences of eukaryotic protein coding genes was carried out using the GENBANK databank. Nucleotide frequencies obtained for the highly conserved regions around the splice sites for different categories of organisms closely agree with each other. A striking similarity among the rare splice junctions which do not contain AG at the 3' splice site or GT at the 5' splice site indicates the existence of special mechanisms to recognize them, and that these unique signals may be involved in crucial gene-regulation events and in differentiation. A method was developed to predict potential exons in a bare sequence, using a scoring and ranking scheme based on nucleotide weight tables. This method was used to find a majority of the exons in selected known genes, and also predicted potential new exons which may be used in alternative splicing situations.

MeSH Terms
Animals Genes Genes, Immunoglobulin Humans Information Systems Molecular Sequence Data Proteins/genetics RNA Splicing RNA, Messenger/genetics Receptors, Antigen, T-Cell/genetics Species Specificity Transcription, Genetic
Chemicals
Proteins RNA, Messenger Receptors, Antigen, T-Cell
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shapiro M B
Laboratory of Statistical and Mathematical Methodology, National Institutes of Health, Bethesda, MD 20892.
Senapathy P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.17.7155
Published
1987-09-11
Pages
7155-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306199
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.17.7155 PMC Full Text View journal details
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