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PMID: 6328447 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The nucleotide sequence of the chicken thymidine kinase gene and the relationship of its predicted polypeptide to that of the vaccinia virus thymidine kinase.

Nucleic acids research ·Vol. 12 ·No. 9 ·1984-05-11 ·Pages 3959-71

Kwoh TJ, Engler JA

Abstract

The entire DNA nucleotide sequence of a 3.0 kilobase pair Hind III fragment containing the chicken cytoplasmic thymidine kinase gene was determined. Oligonucleotide linker insertion mutations distributed throughout this gene and having known effects upon gene activity ( Kwoh , T.J., Zipser , D., and Wigler , M. 1983. J. Mol. Appl. Genet. 2, 191-200), were used to access regions of the Hind III fragment for sequencing reactions. The complete nucleotide sequence, together with the positions of the linker insertion mutations within the sequence, allows us to propose a structure for the chicken thymidine kinase gene. The protein coding sequence of the gene is divided into seven small segments (each less than 160 base pairs) by six small introns (each less than 230 base pairs). The proposed 244 amino acid polypeptide encoded by this gene bears strong homology to the vaccinia virus thymidine kinase. No homology with the thymidine kinases of the herpes simplex viruses was found.

MeSH Terms
Amino Acid Sequence Animals Base Composition Base Sequence Chickens DNA Restriction Enzymes Genes Genes, Viral Mutation Plasmids Species Specificity Thymidine Kinase/genetics Vaccinia virus/genetics
Chemicals
Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kwoh T J
Engler J A
References (45)
45 references, click to expand
  1. Variations in phosphokinase activities during the cell cycle in synchronous populations of HeLa cells.
    Nature. 1965 Jul 10;207(993):176-7 PMID: 5886110
  2. Temporal coordination of DNA replication with enzyme synthesis in diploid and heteroploid cells.
    Science. 1969 Dec 19;166(3912):1536-8 PMID: 17655055
  3. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  4. Deoxythymidine kinase induced in HeLa TK- cells by herpes simplex virus type I and type II. II. Purification and characterization.
    J Biol Chem. 1976 May 10;251(9):2605-10 PMID: 177418
  5. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  6. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.
    Cell. 1977 May;11(1):223-32 PMID: 194704
  7. Biochemical transformation of mouse cells by fragments of herpes simplex virus DNA.
    Cell. 1977 May;11(1):233-41 PMID: 194705
  8. Sequence data handling by computer.
    Nucleic Acids Res. 1977 Nov;4(11):4037-51 PMID: 593900
  9. Association of thymidylate kinase activity with pyrimidine deoxyribonucleoside kinase induced by herpes simplex virus.
    J Biol Chem. 1978 Mar 10;253(5):1325-7 PMID: 203589
  10. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  11. Transformation of mammalian cells with genes from procaryotes and eucaryotes.
    Cell. 1979 Apr;16(4):777-85 PMID: 222468
  12. Computer programs for the assembly of DNA sequences.
    Nucleic Acids Res. 1979 Sep 25;7(2):529-45 PMID: 493154
  13. Hybrid plasmids containing an active thymidine kinase gene of Herpes simplex virus 1.
    Nucleic Acids Res. 1979 Oct 25;7(4):859-77 PMID: 228250
  14. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  15. Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of Herpes simplex type 1 virus.
    Gene. 1979 Nov;7(3-4):335-42 PMID: 230124
  16. Location and cloning of the herpes simplex virus type 2 thymidine kinase gene.
    J Virol. 1980 Mar;33(3):1221-4 PMID: 6245273
  17. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  18. Isolation of the chicken thymidine kinase gene by plasmid rescue.
    Nature. 1980 May 22;285(5762):207-10 PMID: 6246445
  19. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland.
    Cell. 1980 Aug;21(1):179-87 PMID: 6157477
  20. Cloning and sequence analysis of cDNAs encoding two distinct somatostatin precursors found in the endocrine pancreas of anglerfish.
    Nature. 1980 Nov 13;288(5787):137-41 PMID: 6107860
  21. Exons encode functional and structural units of chicken lysozyme.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5759-63 PMID: 6934509
  22. Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro.
    Nucleic Acids Res. 1980 Dec 20;8(24):5931-48 PMID: 6258155
  23. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.
    Nucleic Acids Res. 1980 Dec 20;8(24):5949-64 PMID: 6258156
  24. The structure of eight distinct cloned human leukocyte interferon cDNAs.
    Nature. 1981 Mar 5;290(5801):20-6 PMID: 6163083
  25. Skeletal muscle actin mRNA. Characterization of the 3' untranslated region.
    Nucleic Acids Res. 1981 Feb 11;9(3):579-89 PMID: 6894330
  26. Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1441-5 PMID: 6262799
  27. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  28. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
    Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 PMID: 7301588
  29. Mapping functional domains in the promoter region of the herpes thymidine kinase gene.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6276-80 PMID: 6273861
  30. Processed genes: a dispersed human immunoglobulin gene bearing evidence of RNA-type processing.
    Nature. 1982 Mar 25;296(5855):321-5 PMID: 6801526
  31. Computer programs for nucleic acid sequence manipulation.
    Nucleic Acids Res. 1982 Jan 11;10(1):91-101 PMID: 6278414
  32. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  33. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  34. The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element.
    Nature. 1982 Nov 25;300(5890):330-5 PMID: 6927856
  35. Functional relationships between transcriptional control signals of the thymidine kinase gene of herpes simplex virus.
    Cell. 1982 Dec;31(2 Pt 1):355-65 PMID: 6297762
  36. The nucleotide sequence of mRNA for the Mr 19 000 glycoprotein from early gene block III of adenovirus 2.
    Gene. 1982 Dec;20(3):339-46 PMID: 6985487
  37. Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations.
    J Virol. 1983 May;46(2):530-7 PMID: 6842679
  38. Nucleotide sequence of the herpes simplex virus type 2 thymidine kinase gene.
    J Virol. 1983 Jun;46(3):1045-50 PMID: 6304336
  39. Fine structure analysis and nucleotide sequence of the vaccinia virus thymidine kinase gene.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3411-5 PMID: 6304709
  40. Mutational analysis of the cloned chicken thymidine kinase gene.
    J Mol Appl Genet. 1983;2(2):191-200 PMID: 6875426
  41. Molecular cloning and cell cycle-specific regulation of a functional human thymidine kinase gene.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5588-91 PMID: 6577446
  42. Genomic cloning and preliminary characterization of the human thymidine kinase gene.
    Proc Natl Acad Sci U S A. 1983 Nov;80(21):6528-32 PMID: 6314329
  43. Isolation and preliminary characterization of the Chinese hamster thymidine kinase gene.
    Mol Cell Biol. 1983 Oct;3(10):1815-23 PMID: 6227808
  44. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.
    Science. 1964 Aug 14;145(3633):709-10 PMID: 14168277
  45. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.9.3959
Published
1984-05-11
Pages
3959-71
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318803
Subset
IM
Grants
NCI NIH HHS · CA-1314812 · United States
Databases
GENBANK
X00571
External Links
PubMed source DOI: 10.1093/nar/12.9.3959 PMC Full Text View journal details
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