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PMID: 6314329 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genomic cloning and preliminary characterization of the human thymidine kinase gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 21 ·1983-11-00 ·Pages 6528-32

Lin PF, Zhao SY, Ruddle FH

Abstract

In this report, we describe the cloning of the human cytoplasmic thymidine kinase (tk-C; EC 2.7.1.21) gene and its preliminary characterization. The tk-C sequences were isolated from a phage genomic library made from DNA of a transfected mouse cell carrying the human tk-C gene. The human transforming sequences were identified by homology with human Alu sequences. Six recombinant phages were isolated and five were competent to transfer human TK-C activity to TK-deficient mouse cells when transferred in pairs. Conclusively, sequences homologous to these clones are present in all human TK+ transformants examined. We estimate the maximal size of the tk-C gene to be 14 kilobase pairs and its minimal size to be between 4 and 5 kilobase pairs. The gene contains many noncoding inserts and numerous Alu sequences.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes Genes Humans Repetitive Sequences, Nucleic Acid Thymidine Kinase/genetics Transformation, Genetic
Chemicals
Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lin P F
Zhao S Y
Ruddle F H
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.21.6528
Published
1983-11-00
Pages
6528-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390386
Subset
IM
Grants
NIGMS NIH HHS · GM09966 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.21.6528 PMC Full Text View journal details
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