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PMID: 22018597 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Clathrin-mediated endocytosis in budding yeast.

Trends in cell biology ·Vol. 22 ·No. 1 ·2012-01-00 ·Pages 1-13

Weinberg J, Drubin DG

Abstract

Clathrin-mediated endocytosis in the budding yeast Saccharomyces cerevisiae involves the ordered recruitment, activity and disassembly of nearly 60 proteins at distinct sites on the plasma membrane. Two-color live-cell fluorescence microscopy has proven to be invaluable for in vivo analysis of endocytic proteins: identifying new components, determining the order of protein arrival and dissociation, and revealing even very subtle mutant phenotypes. Yeast genetics and functional genomics facilitate identification of complex interaction networks between endocytic proteins and their regulators. Quantitative datasets produced by these various analyses have made theoretical modeling possible. Here, we discuss recent findings on budding yeast endocytosis that have advanced our knowledge of how -60 endocytic proteins are recruited, perform their functions, are regulated by lipid and protein modifications, and are disassembled, all with remarkable regularity.

MeSH Terms
Animals Clathrin/pharmacology Endocytosis/drug effects Genome, Fungal Humans Protein Processing, Post-Translational Saccharomyces cerevisiae/drug effects,genetics Selection, Genetic
Chemicals
Clathrin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weinberg Jasper
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202, USA.
Drubin David G
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Article Info
Journal
Trends in cell biology
Abbr.
Trends Cell Biol
ISSN
1879-3088
DOI
10.1016/j.tcb.2011.09.001
Published
2012-01-00
Epub
2011-00-20
Pages
1-13
Language
English
Region
England
NLM ID
9200566
PMCID
PMC3253927
Subset
IM
Grants
NIGMS NIH HHS · R01 GM042759 · United States
NIGMS NIH HHS · R01 GM050399-16 · United States
NIGMS NIH HHS · GM R01 50399 · United States
NIGMS NIH HHS · R01 GM042759-17 · United States
NIGMS NIH HHS · R01 GM050399 · United States
NIGMS NIH HHS · GM R01 42759 · United States
External Links
PubMed source DOI: 10.1016/j.tcb.2011.09.001 PMC Full Text View journal details
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